CIBERDEM, Hospital Universitari Joan XXIII, IISPV, Tarragona, Spain.
PLoS One. 2012;7(2):e31280. doi: 10.1371/journal.pone.0031280. Epub 2012 Feb 23.
De novo lipogenesis is involved in fatty acid biosynthesis and could be involved in the regulation of the triglyceride storage capacity of adipose tissue. However, the association between lipogenic and lipolytic genes and the evolution of morbidly obese subjects after bariatric surgery remains unknown. In this prospective study we analyze the association between the improvement in the morbidly obese patients as a result of bariatric surgery and the basal expression of lipogenic and lipolytic genes.
We study 23 non diabetic morbidly obese patients who were studied before and 7 months after bariatric surgery. Also, we analyze the relative basal mRNA expression levels of lipogenic and lipolytic genes in epiploic visceral adipose tissue (VAT) and abdominal subcutaneous adipose tissue (SAT).
When the basal acetyl-CoA carboxylase 1 (ACC1), acetyl-CoA synthetase 2 (ACSS2) and ATP citrate lyase (ACL) expression in SAT was below percentile-50, there was a greater decrease in weight (P = 0.006, P = 0.034, P = 0.026), body mass index (P = 0.008, P = 0.033, P = 0.034) and hip circumference (P = 0.033, P = 0.021, P = 0.083) after bariatric surgery. In VAT, when the basal ACSS2 expression was below percentile-50, there was a greater decrease in hip circumference (P = 0.006). After adjusting for confounding variables in logistic regression models, only the morbidly obese patients with SAT or VAT ACSS2 expression ≥ P50 before bariatric surgery had a lower percentage hip circumference loss (<P50) after bariatric surgery (SAT: P = 0.039; VAT: P = 0.033).
A lower basal ACSS2, ACC1 and ACL expression, genes involved in de novo lipogenesis, is associated with a better evolution of anthropometric variables after bariatric surgery. Thus, the previous state of the pathways involved in fatty acid metabolism may have repercussions on the improvement of these patients.
从头合成脂肪酸参与脂肪酸的生物合成，并可能参与调节脂肪组织的甘油三酯储存能力。然而，脂肪生成和脂肪分解基因与肥胖症患者在接受减肥手术后的演变之间的关系尚不清楚。在这项前瞻性研究中，我们分析了肥胖症患者因减肥手术而改善与脂肪生成和脂肪分解基因基础表达之间的关系。
我们研究了 23 名非糖尿病肥胖症患者，这些患者在减肥手术前和手术后 7 个月进行了研究。此外，我们还分析了内脏网膜脂肪组织（VAT）和腹部皮下脂肪组织（SAT）中脂肪生成和脂肪分解基因的相对基础 mRNA 表达水平。
当 SAT 中的基础乙酰辅酶 A 羧化酶 1（ACC1）、乙酰辅酶 A 合成酶 2（ACSS2）和三磷酸柠檬酸裂解酶（ACL）表达低于第 50 个百分位数时，体重（P=0.006，P=0.034，P=0.026）、体重指数（P=0.008，P=0.033，P=0.034）和臀围（P=0.033，P=0.021，P=0.083）在减肥手术后下降更大。在 VAT 中，当基础 ACSS2 表达低于第 50 个百分位数时，臀围下降更大（P=0.006）。在逻辑回归模型中调整混杂变量后，只有在减肥手术前 SAT 或 VAT 中 ACSS2 表达≥P50 的肥胖症患者，在减肥手术后其臀围百分比下降幅度较小（<P50）（SAT：P=0.039；VAT：P=0.033）。
从头合成脂肪酸生成相关基因基础表达的降低，即 ACSS2、ACC1 和 ACL，与减肥手术后人体测量学变量的更好演变相关。因此，脂肪酸代谢相关途径的先前状态可能对这些患者的改善产生影响。
