Laboratory of Endocrinology and Genomics, CHUL Research Center CHUQ, Laval University, Québec City, Québec, Canada.
PLoS One. 2012;7(2):e31532. doi: 10.1371/journal.pone.0031532. Epub 2012 Feb 24.
T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although these studies reported the proteomic profiles of each cell line, until now, their differential protein expression profiles have not been established. Here, we used two-dimensional gel and mass spectrometry analyses to compare the proteomic profiles of the two cell lines, T47D and MCF7. Our data revealed that more than 164 proteins are differentially expressed between them. According to their biological functions, the results showed that proteins involved in cell growth stimulation, anti-apoptosis mechanisms and cancerogenesis are more strongly expressed in T47D than in MCF7. These proteins include G1/S-specific cyclin-D3 and prohibitin. Proteins implicated in transcription repression and apoptosis regulation, including transcriptional repressor NF-X1, nitrilase homolog 2 and interleukin-10, are, on the contrary, more strongly expressed in MCF7 as compared to T47D. Five proteins that were previously described as breast cancer biomarkers, namely cathepsin D, cathepsin B, protein S100-A14, heat shock protein beta-1 (HSP27) and proliferating cell nuclear antigen (PCNA), are found to be differentially expressed in the two cell lines. A list of differentially expressed proteins between T47D and MCF7 was generated, providing useful information for further studies of breast cancer mechanisms with these cell lines as models.
T47D 和 MCF7 是两种人激素依赖性乳腺癌细胞系，广泛用于体外和体内（肿瘤异种移植）乳腺癌研究的实验模型。通过蛋白质组学分析鉴定了这两种细胞系中几种与癌症发展有关的蛋白质。尽管这些研究报告了每种细胞系的蛋白质组图谱，但到目前为止，它们的差异蛋白质表达图谱尚未建立。在这里，我们使用二维凝胶和质谱分析来比较 T47D 和 MCF7 这两种细胞系的蛋白质组图谱。我们的数据显示，它们之间有超过 164 种蛋白质表达存在差异。根据它们的生物学功能，结果表明，参与细胞生长刺激、抗凋亡机制和癌症发生的蛋白质在 T47D 中的表达比在 MCF7 中更强。这些蛋白质包括 G1/S 特异性细胞周期蛋白 D3 和抑制素。参与转录抑制和凋亡调节的蛋白质，包括转录抑制因子 NF-X1、亚硝酰酶同源物 2 和白细胞介素 10，与 T47D 相比，在 MCF7 中表达更强。有 5 种先前被描述为乳腺癌生物标志物的蛋白质，即组织蛋白酶 D、组织蛋白酶 B、S100-A14 蛋白、热休克蛋白 beta-1（HSP27）和增殖细胞核抗原（PCNA），在这两种细胞系中表达存在差异。生成了 T47D 和 MCF7 之间差异表达的蛋白质列表，为使用这些细胞系作为模型进一步研究乳腺癌机制提供了有用的信息。
