Thirkell D, Spooner R K, Jones G E, Russell W C
Department of Biochemistry and Microbiology, University of St. Andrews, Fife, Great Britain.
Vet Microbiol. 1990 Aug;24(2):143-53. doi: 10.1016/0378-1135(90)90062-z.
Using sera from lambs experimentally infected with Mycoplasma ovipneumoniae and Pasteurella haemolytica, the development of a good humoral immune response to M. ovipneumoniae was detected by ELISA. The antibody titres peaked 41 days post-infection and good antibody titres were maintained over the 16-week experimental period. Immunoblotting revealed that antibodies to specific antigens appeared in the sera in a sequential manner, some being seen shortly after infection and others developing only after a substantial time lag. Antibodies were raised against almost all the major antigens detected in one laboratory strain (956/2) and against all antigens previously shown to be conserved in 22 Scottish field isolates of M. ovipneumoniae.
