Niang M, Rosenbusch R F, Andrews J J, Lopez-Virella J, Kaeberle M L
Department of Microbiology, Immunology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Vet Immunol Immunopathol. 1998 Jul 31;64(3):191-205. doi: 10.1016/s0165-2427(98)00133-0.
A respiratory disease of lambs that has been termed the 'coughing syndrome' has been observed in the mid-western region of the United States of America. Mycoplasma ovipneumoniae (M. ovipneumoniae) and Mycoplasma arginini (M. arginini) were routinely isolated from the respiratory tract of lambs with this disease. A high level of antibodies reactive with ovine cilia of the upper respiratory tract was detected in the sera from many of the lambs in affected flocks but not in sera of lambs from unaffected flocks. The reactivity of these antibodies with cilia was demonstrated by ELISA and confirmed by indirect immunofluorescent staining and western immunoblotting. These antibodies were predominantly of the IgG isotype. They were distinct from cold or warm agglutinins and could be absorbed from the sera with cilia but not with antigens of common bacterial pathogens of the sheep respiratory tract including M. ovipneumoniae, M. arginini, Pasteurella haemolytica, Pasteurella multocida or Neisseria ovis. In addition, their occurrence appeared to be independent of the specific antibodies to M. ovipneumoniae and M. arginini. Western immunoblotting indicated that the antibodies were directed primarily against an antigen with apparent molecular weight of 50 kDa. In one flock from which serial serum samples were collected from the same lambs over a 10-month period, antibodies to ovine cilia developed before the onset of the clinical disease and persisted for a period of several months until most of the lambs had apparently recovered. However, colonization of the respiratory tract of the lambs by M. ovipneumoniae preceded the production of these antibodies. Sequential serum samples taken from another flock, with no known history of this coughing, showed no such antibodies throughout the sampling period. It is suggested that an immunopathologic mechanism involving production of autoantibodies directed against a ciliary antigen of the lambs could be a contributing factor to the pathogenesis of this clinical disease.
在美国中西部地区观察到一种被称为“咳嗽综合征”的羔羊呼吸道疾病。绵羊肺炎支原体（M. ovipneumoniae）和精氨酸支原体（M. arginini）经常从患有这种疾病的羔羊呼吸道中分离出来。在受影响羊群中许多羔羊的血清中检测到与上呼吸道羊纤毛反应的高水平抗体，但在未受影响羊群的羔羊血清中未检测到。通过酶联免疫吸附测定（ELISA）证明了这些抗体与纤毛的反应性，并通过间接免疫荧光染色和免疫印迹法进行了确认。这些抗体主要是IgG同种型。它们与冷或热凝集素不同，可以用纤毛从血清中吸收，但不能用绵羊呼吸道常见细菌病原体的抗原吸收，包括绵羊肺炎支原体、精氨酸支原体、溶血巴斯德氏菌、多杀巴斯德氏菌或绵羊奈瑟氏菌。此外，它们的出现似乎与针对绵羊肺炎支原体和精氨酸支原体的特异性抗体无关。免疫印迹法表明，这些抗体主要针对一种表观分子量为50 kDa的抗原。在一个羊群中，在10个月的时间里从同一批羔羊中采集了系列血清样本，针对羊纤毛的抗体在临床疾病发作前就已出现，并持续了几个月，直到大多数羔羊明显康复。然而，绵羊肺炎支原体在羔羊呼吸道的定植先于这些抗体的产生。从另一个没有这种咳嗽病史的羊群中采集的系列血清样本在整个采样期间均未显示出此类抗体。有人提出，涉及产生针对羔羊纤毛抗原的自身抗体的免疫病理机制可能是这种临床疾病发病机制的一个促成因素。
