玉米条纹病毒主要非衣壳蛋白基因的鉴定与序列分析

Identification and sequence analysis of the maize stripe virus major noncapsid protein gene.

作者信息

Huiet L, Klaassen V, Tsai J H, Falk B W

机构信息

Department of Plant Pathology, University of California, Davis 95616.

出版信息

Virology. 1990 Dec;179(2):862-6. doi: 10.1016/0042-6822(90)90156-l.

DOI:10.1016/0042-6822(90)90156-l

PMID:2238475

Abstract

The maize stripe virus (MStV) major noncapsid protein (NCP) gene was characterized, and the location of the NCP gene was identified among the 5-RNA, 18-kb genome. A 12-amino-acid sequence of the NCP was compared with nucleotide sequence data for MStV RNAs 3 and 4 and was found to align perfectly within a 528-nucleotide open reading frame (ORF) of RNA 4. The amino acid composition of purified NCP was almost identical to that deduced from the putative coding region. The deduced NCP molecular weight was 19,815, very similar to that determined by SDS-PAGE analysis of the purified NCP. In vitro transcription and translation analysis of the cDNA representing this region showed unequivocally that this region encoded the NCP. Primer extension analysis using a synthetic oligonucleotide complementary to a sequence near the 5' end of the coding region revealed that the NCP ORF is located 61 nucleotides from the 5' end of RNA 4.

摘要

对玉米条纹病毒（MStV）主要非衣壳蛋白（NCP）基因进行了表征，并在18 kb的5-RNA基因组中确定了NCP基因的位置。将NCP的12个氨基酸序列与MStV RNA 3和4的核苷酸序列数据进行比较，发现其与RNA 4的528个核苷酸开放阅读框（ORF）完全对齐。纯化的NCP的氨基酸组成与从推定编码区推导的氨基酸组成几乎相同。推导的NCP分子量为19,815，与通过纯化的NCP的SDS-PAGE分析确定的分子量非常相似。对代表该区域的cDNA进行的体外转录和翻译分析明确表明该区域编码NCP。使用与编码区5'端附近序列互补的合成寡核苷酸进行引物延伸分析表明，NCP ORF位于RNA 4 5'端61个核苷酸处。