Chirkov Iu Iu, Tyshchiuk I A, Severina I S, Starosel'tseva L K
Vopr Med Khim. 1990 Jul-Aug;36(4):20-2.
Dilution of thrombocyte-rich blood plasma (down to 2.5 x 10(8) thrombocytes/ml) enabled to avoid an irreversible phase of the ADP-induced thrombocyte aggregation; the reversible phase of aggregation was followed by deaggregation. The reversible aggregation of thrombocytes was studied in healthy persons and patients with diabetes mellitus within wide ranges of ADP concentrations (including 10 microM) using the diluted blood plasma. Thrombocytes of the patients with diabetes mellitus of the I and II types were found to be 1.6- and 2.33-fold more sensitive to the ADP aggregating effect as compared with thrombocytes of healthy persons. The elevated rate of thrombocytes aggregation in diabetes mellitus appears to be related to early decrease in activity of thrombocyte guanylate cyclase and to its ability to be activated.
富含血小板血浆的稀释（稀释至2.5×10⁸个血小板/毫升）能够避免ADP诱导的血小板聚集的不可逆阶段；聚集的可逆阶段之后是解聚。使用稀释血浆在广泛的ADP浓度范围（包括10微摩尔）内研究了健康人和糖尿病患者血小板的可逆聚集。发现I型和II型糖尿病患者的血小板对ADP聚集作用的敏感性分别是健康人血小板的1.6倍和2.33倍。糖尿病中血小板聚集率升高似乎与血小板鸟苷酸环化酶活性早期降低及其被激活的能力有关。
