Halushka P V, Lurie D, Colwell J A
N Engl J Med. 1977 Dec 15;297(24):1306-10. doi: 10.1056/NEJM197712152972402.
We studied the effects of ADP, epinephrine, collagen and arachidonic acid on platelet production of immunoreactive prostaglandin-E-like material and aggregation in 17 subjects with diabetes mellitus and 21 matched controls. Plateletrich plasma obtained from patients synthesized significantly (P less than 0.05) greater quantities of the prostaglandin-E-like material after exposure to 1 muM ADP, 1, 2 and 5 muM epinephrine and 1 microgram per milliliter of collagen than platelet-rich plasma obtained from controls. That obtained from the diabetic patients was significantly more sensitive (P less than 0.001) to the aggregating effects of the prostaglandin precursor, arachidonic acid, in vitro as compared to controls. Diabetic platelet-rich plasma metabolized arachidonic acid (0.5 mM) to immunoreactive prostaglandin-E-like material at a significantly greater rate (P less than 0.05) and extent (P less than 0.001) than that of controls. Thus, platelets obtained from diabetic patients possess increased activity of the prostaglandin synthetase system, and this characteristic may be related to the increased platelet aggregation associated with the disease.
我们研究了二磷酸腺苷（ADP）、肾上腺素、胶原蛋白和花生四烯酸对17名糖尿病患者及21名配对对照者血小板产生免疫反应性前列腺素E样物质及聚集的影响。与对照组相比，糖尿病患者的富含血小板血浆在暴露于1微摩尔/升ADP、1、2和5微摩尔/升肾上腺素以及每毫升1微克胶原蛋白后，合成的前列腺素E样物质显著增多（P<0.05）。与对照组相比，糖尿病患者的富含血小板血浆在体外对前列腺素前体花生四烯酸的聚集作用明显更敏感（P<0.001）。糖尿病患者的富含血小板血浆将花生四烯酸（0.5毫摩尔/升）代谢为免疫反应性前列腺素E样物质的速率（P<0.05）和程度（P<0.001）均显著高于对照组。因此，糖尿病患者的血小板中前列腺素合成酶系统的活性增强，这一特征可能与该疾病相关的血小板聚集增加有关。
