Vasil'ev A V, Bregvadze N S, Tutel'ian V A
Vopr Med Khim. 1990 Jul-Aug;36(4):45-7.
Activity of lysosomal proteinases cathepsins A, B, C, D, H and L was studied in liver, kidney, spleen, thymus, heart, skeletal muscle and brain tissues of rats, maintained on full value diet, on a diet free of proteins and on the diet enriched with methionine. Distinctly dissimilar reactions of thiol-dependent proteinases was detected in liver, heart tissues and skeletal muscles as compared with the reaction of cathepsins A and D; activity of cathepsins B, H and L was decreased 1.5-2-fold in the animal groups maintained on diets both free of proteins and enriched with methionine. The data obtained suggest that concentration of endogenous inhibitors of lysosomal thiol-dependent proteinases rather than content of SH-groups in tissues was responsible for alterations of their activity under conditions of protein starvation.
在以全价饲料、无蛋白饲料和富含蛋氨酸的饲料喂养的大鼠的肝脏、肾脏、脾脏、胸腺、心脏、骨骼肌和脑组织中，研究了溶酶体蛋白酶组织蛋白酶A、B、C、D、H和L的活性。与组织蛋白酶A和D的反应相比，在肝脏、心脏组织和骨骼肌中检测到硫醇依赖性蛋白酶有明显不同的反应；在以无蛋白饲料和富含蛋氨酸的饲料喂养的动物组中，组织蛋白酶B、H和L的活性降低了1.5至2倍。所得数据表明，在蛋白质饥饿条件下，溶酶体硫醇依赖性蛋白酶活性的改变是由内源性抑制剂的浓度而非组织中SH基团的含量所致。
