对分泌型和非分泌型个体颊黏膜上皮细胞及唾液中1型和2型H抗原进行半定量测定。

Semi-quantitative determination of H type 1 and type 2 antigens on buccal epithelial cells and in saliva of secretors and non-secretors.

作者信息

Rahat A, Stewart J, Blackwell C C, Weir D M

机构信息

Department of Bacteriology, Medical School, University of Edinburgh, UK.

出版信息

Vox Sang. 1990;59(2):101-5. doi: 10.1111/j.1423-0410.1990.tb05019.x.

DOI:10.1111/j.1423-0410.1990.tb05019.x

PMID:2238560

Abstract

The amount of H antigens on buccal epithelial cells (BEC) of secretors and non-secretors was measured by flow cytometry. H type 1 and H type 2 on BEC were detected with fluorescein-labelled Ulex europaeus lectin, and H type 2 was measured with mouse monoclonal antibody. Between 3.5 and 6 times more H antigen was detected on cells from secretors compared with cells from non-secretors. The level of H type 2 was the same on BEC of secretors and non-secretors. Adsorption of H type 1 antigen from secretor saliva onto non-secretors BEC was demonstrated. A simple and sensitive ELISA method was developed for the determination of H in saliva with biotin-labelled Ulex lectin. This system provides a simple means for measuring the amount of H present in saliva and for the determination of secretor status.

摘要

通过流式细胞术测量分泌型和非分泌型个体颊黏膜上皮细胞（BEC）上H抗原的量。用荧光素标记的荆豆凝集素检测BEC上的1型H和2型H，并用小鼠单克隆抗体测量2型H。与非分泌型个体的细胞相比，在分泌型个体的细胞上检测到的H抗原多3.5至6倍。分泌型和非分泌型个体的BEC上2型H的水平相同。证实了分泌型唾液中的1型H抗原可吸附到非分泌型个体的BEC上。开发了一种简单而灵敏的酶联免疫吸附测定（ELISA）方法，用生物素标记的荆豆凝集素来测定唾液中的H。该系统为测量唾液中H的含量和确定分泌型状态提供了一种简单的方法。