Zorgani A A, Stewart J, Blackwell C C, Elton R A, Weir D M
Department of Medical Microbiology, University Medical School, Edinburgh, UK.
FEMS Immunol Med Microbiol. 1994 Aug;9(2):135-42. doi: 10.1111/j.1574-695X.1994.tb00484.x.
Carriage of Neisseria meningitidis B:4:P1.15 was higher among non-secretors during a school outbreak of meningitis; non-secretors had lower levels of anti-meningococcal salivary IgM. Flow cytometry was used to assess effects of secretor and non-secretor saliva on binding of B:4:P1.15 to buccal epithelial cells: (1) to assess inhibition by IgA and IgM; and (2) to assess contributions of salivary antibodies to inhibitory activities. Greater inhibition was obtained with secretor saliva: pooled (P = 0.049); fresh (P = 0.0001). Purified IgA (P = 0.02) and IgM (P = 0.03) were equally inhibitory. After absorption of anti-meningococcal antibodies, there was still significant inhibitory activity in the pools: secretors (P = 0.018); non-secretors (P = 0.005). These results indicate that both secretory immunoglobulins and other factors contribute to protection against colonisation by meningococci and might explain the increased carriage of B:4:1.15 in this population.
在一次学校脑膜炎暴发期间，B:4:P1.15型脑膜炎奈瑟菌的携带率在非分泌型人群中更高；非分泌型人群的抗脑膜炎球菌唾液IgM水平较低。采用流式细胞术评估分泌型和非分泌型唾液对B:4:P1.15与颊上皮细胞结合的影响：（1）评估IgA和IgM的抑制作用；（2）评估唾液抗体对抑制活性的贡献。分泌型唾液具有更强的抑制作用：混合唾液（P = 0.049）；新鲜唾液（P = 0.0001）。纯化的IgA（P = 0.02）和IgM（P = 0.03）具有同等的抑制作用。在去除抗脑膜炎球菌抗体后，混合唾液中仍存在显著的抑制活性：分泌型（P = 0.018）；非分泌型（P = 0.005）。这些结果表明，分泌型免疫球蛋白和其他因素均有助于预防脑膜炎球菌定植，这可能解释了该人群中B:4:1.15型脑膜炎奈瑟菌携带率增加的原因。
