Department of Pathology, Kuo General Hospital, Tainan, Taiwan.
Kaohsiung J Med Sci. 2012 Mar;28(3):145-50. doi: 10.1016/j.kjms.2011.10.004. Epub 2012 Jan 18.
This study investigates the expression of annexin 1 in urothelial carcinoma (UC) and its relation with clinicopathologic factors, and evaluates its potential clinical significance. Annexin 1 expression was analyzed by immunohistochemical staining with manual tissue microarrays and Western blot in UC. Immunohistochemical analysis of UC in tissue microarrays showed that annexin 1 protein was 76.5% (150/196) positive, which was markedly increased compared with that in the normal urothelium 20.8% (5/24) (p < 0.01). In addition, the positive expression rate of annexin 1 was higher in the high-grade UC (81.7%; 143/175) than in the low-grade UC (33.3%; 7/21). Western blot revealed that the expression of annexin 1 was low in low-grade UC, and markedly increased in high-grade UC. In conclusion, annexin 1 overexpression is observed in UC, which suggests it may be associated with tumorigenesis and its expression correlates with the differentiation of UC.
本研究旨在探讨膜联蛋白 1 在尿路上皮癌（UC）中的表达及其与临床病理因素的关系，并评估其潜在的临床意义。采用组织微阵列免疫组织化学染色和 Western blot 检测膜联蛋白 1 的表达。组织微阵列中 UC 的免疫组织化学分析显示，膜联蛋白 1 蛋白阳性率为 76.5%（150/196），明显高于正常尿路上皮的 20.8%（5/24）（p<0.01）。此外，膜联蛋白 1 的阳性表达率在高级别 UC 中（81.7%；143/175）高于低级别 UC（33.3%；7/21）。Western blot 显示膜联蛋白 1 在低级别 UC 中表达较低，在高级别 UC 中表达显著增加。综上所述，UC 中存在膜联蛋白 1 的过表达，提示其可能与肿瘤发生有关，其表达与 UC 的分化相关。
