Department of Medical Oncology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029 Nanjing, PR China.
Biomed Pharmacother. 2012 Mar;66(2):144-50. doi: 10.1016/j.biopha.2011.11.021. Epub 2012 Feb 17.
CD44 molecule plays critical role in distant malignant metastasis. It is expressed in standard form (CD44s) or variant form (CD44v). Tumor necrosis factor-α (TNF-α) is highly expressed in the cancer microenvironment. TNF-α was reported to modulate CD44 expression in several kinds of cancer. However, little is known about pathological role of TNF-α in breast cancer (BC) cells. In the current investigation, we investigated the effect of TNF-α on BC cells (MCF-7 and MDA-MB-231) viability, CD44 expression, and in vitro migration. We found that TNF-α down-regulated CD44s expression, up-regulated CD44v3 and CD44v6 expression through JNK pathway in MCF-7 cells. In MDA-MB-231 cells, TNF-α up-regulated CD44s, CD44v3 and CD44v6 expression via p38 pathway. These data indicate important role of CD44 molecule in BC pathology.
CD44 分子在远处恶性转移中起着关键作用。它以标准形式（CD44s）或变体形式（CD44v）表达。肿瘤坏死因子-α（TNF-α）在癌症微环境中高度表达。据报道，TNF-α在几种癌症中调节 CD44 的表达。然而，TNF-α在乳腺癌（BC）细胞中的病理作用知之甚少。在目前的研究中，我们研究了 TNF-α对 BC 细胞（MCF-7 和 MDA-MB-231）活力、CD44 表达和体外迁移的影响。我们发现 TNF-α通过 JNK 途径下调 MCF-7 细胞中 CD44s 的表达，上调 CD44v3 和 CD44v6 的表达。在 MDA-MB-231 细胞中，TNF-α通过 p38 途径上调 CD44s、CD44v3 和 CD44v6 的表达。这些数据表明 CD44 分子在 BC 病理学中起着重要作用。
