Institute of Vegetables, Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Improvement Center, Jinan 250100, China.
Genomics. 2012 May;99(5):299-307. doi: 10.1016/j.ygeno.2012.02.005. Epub 2012 Feb 23.
In this study, we report the first use of RNA-sequencing to gain insight into the wide range of transcriptional events that are associated with leafy head development in Chinese cabbage. We generated 53.5 million sequence reads (90 bp in length) from the rosette and heading leaves. The sequence reads were aligned to the recently sequenced Chiifu genome and were analyzed to measure the gene expression levels, to detect alternative splicing events and novel transcripts, to determine the expression of single nucleotide polymorphisms, and to refine the annotated gene structures. The analysis of the global gene expression pattern suggests two important concepts, which govern leafy head formation. Firstly, some stimuli, such as carbohydrate levels, light intensity and endogenous hormones might play a critical role in regulating the leafy head formation. Secondly, the regulation of transcription factors, protein kinases and calcium may also be involved in this developmental process.
在这项研究中，我们首次利用 RNA 测序深入了解与大白菜结球形成相关的广泛转录事件。我们从莲座叶和结球叶中生成了 5350 万条序列（长度为 90bp）。将这些序列与最近测序的 Chiifu 基因组进行比对，并进行分析以测量基因表达水平、检测选择性剪接事件和新的转录本、确定单核苷酸多态性的表达以及细化注释基因结构。全局基因表达模式的分析提出了两个重要概念，它们控制着结球的形成。首先，一些刺激物，如碳水化合物水平、光强度和内源性激素，可能在调节结球形成中发挥关键作用。其次，转录因子、蛋白激酶和钙的调节也可能参与这个发育过程。
