Guo Xinlei, Liang Jianli, Lin Runmao, Zhang Lupeng, Wu Jian, Wang Xiaowu
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2022 Jan 6;12:787826. doi: 10.3389/fpls.2021.787826. eCollection 2021.
Chinese cabbage is an important leaf heading vegetable crop. At the heading stage, its leaves across inner to outer show significant morphological differentiation. However, the genetic control of this complex leaf morphological differentiation remains unclear. Here, we reported the transcriptome profiling of Chinese cabbage plant at the heading stage using 24 spatially dissected tissues representing different regions of the inner to outer leaves. Genome-wide transcriptome analysis clearly separated the inner leaf tissues from the outer leaf tissues. In particular, we identified the key transition leaf by the spatial expression analysis of key genes for leaf development and sugar metabolism. We observed that the key transition leaves were the first inwardly curved ones. Surprisingly, most of the heading candidate genes identified by domestication selection analysis obviously showed a corresponding expression transition, supporting that key transition leaves are related to leafy head formation. The key transition leaves were controlled by a complex signal network, including not only internal hormones and protein kinases but also external light and other stimuli. Our findings provide new insights and the rich resource to unravel the genetic control of heading traits.
大白菜是一种重要的结球叶菜类作物。在结球期，其从内层到外层的叶片呈现出显著的形态分化。然而，这种复杂的叶片形态分化的遗传控制仍不清楚。在此，我们报道了利用代表内层到外层叶片不同区域的24个空间解剖组织对大白菜结球期植株进行的转录组分析。全基因组转录组分析清楚地将内层叶组织与外层叶组织区分开来。特别是，我们通过对叶片发育和糖代谢关键基因的空间表达分析确定了关键过渡叶。我们观察到关键过渡叶是最初向内弯曲的叶片。令人惊讶的是，通过驯化选择分析鉴定出的大多数结球候选基因明显表现出相应的表达转变，这支持了关键过渡叶与叶球形成有关。关键过渡叶受一个复杂的信号网络控制，该网络不仅包括内部激素和蛋白激酶，还包括外部光照和其他刺激。我们的研究结果为揭示结球性状的遗传控制提供了新的见解和丰富的资源。
