Korean Agricultural Culture Collection (KACC), National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1291-1296. doi: 10.1099/ijs.0.043455-0. Epub 2012 Jul 13.
A bacterial strain, designated HOR6-4(T), was isolated from the epidermal tissue of a root of a Phalaenopsis orchid. Strain HOR6-4(T) was characterized using a polyphasic approach. The cells were aerobic, Gram-stain-positive, non-motile coccoids. Phylogenetic analysis of its 16S rRNA gene sequence revealed a clear affiliation with the family Dermacoccaceae. Strain HOR6-4(T) showed relatively low 16S rRNA gene sequence similarity (below 95.9 %) to type strains of species with validly published names, showing the highest sequence similarities to the type strains of Flexivirga alba (95.8 %) and Yimella lutea (95.5 %). The DNA G+C content of strain HOR6-4(T) was 64.7 mol%. Strain HOR6-4(T) had anteiso-C17 : 0 (19.3 %), 10-methyl C18 : 0 (tuberculostearic acid; 13.5 %) and 10-methyl C17 : 0 (11.7 %) as the major fatty acids and contained MK-8(H4) and MK-8(H6) as the predominant quinones. Polar lipids were diphosphatidylglycerol, phosphatidylinositol, three aminophospholipids, two phospholipids and an aminolipid. The peptidoglycan type was A4α, with an L-Lys-L-Thr-D-Glu interpeptide bridge with a glycine residue bound to the alpha-carboxyl group of D-Glu in position 2 of the peptide subunit. Mycolic acids were not present. On the basis of comparative analysis of phenotypic and phylogenetic traits, strain HOR6-4(T) is considered to represent a novel species in a new genus in the family Dermacoccaceae, for which the name Rudaeicoccus suwonensis gen. nov., sp. nov. is proposed. The type strain of Rudaeicoccus suwonensis is HOR6-4(T) ( = KACC 12637(T) = DSM 19560(T)).
一株细菌菌株，命名为 HOR6-4(T)，从蝴蝶兰兰花根的表皮组织中分离得到。该菌株 HOR6-4(T)通过多相鉴定方法进行了表征。细胞为需氧、革兰氏阳性、非运动球菌。16S rRNA 基因序列的系统发育分析表明，其与 Dermacoccaceae 家族密切相关。菌株 HOR6-4(T)与具有有效发表名称的物种的模式株的 16S rRNA 基因序列相似性较低（低于 95.9%），与 Flexivirga alba（95.8%）和 Yimella lutea（95.5%）的模式株的序列相似性最高。菌株 HOR6-4(T)的 DNA G+C 含量为 64.7 mol%。菌株 HOR6-4(T)的主要脂肪酸为anteiso-C17 : 0（19.3%）、10-甲基 C18 : 0（结核硬脂酸；13.5%）和 10-甲基 C17 : 0（11.7%），并含有 MK-8(H4)和 MK-8(H6)作为主要醌。极性脂类为双磷脂酰甘油、磷脂酰肌醇、三种氨基磷脂、两种磷脂和一种氨基脂。肽聚糖类型为 A4α，具有 L-Lys-L-Thr-D-Glu 间肽桥，在肽亚基的第 2 位，连接到 D-Glu 的α-羧基的甘氨酸残基。不存在分枝菌酸。基于表型和系统发育特征的比较分析，菌株 HOR6-4(T)被认为代表 Dermacoccaceae 科中新属中的一个新种，建议将其命名为 Rudaeicoccus suwonensis gen. nov.，sp. nov.。Rudaeicoccus suwonensis 的模式菌株为 HOR6-4(T)（=KACC 12637(T)=DSM 19560(T)）。
