Drabik Jacek, Jagiełło Agata, Niemcunowicz-Janica Anna, Pepiński Witold
Z Wydziału Biologii Centralnego Laboratorium Kryminalistycznego KGP w Warszawie.
Arch Med Sadowej Kryminol. 2011 Apr-Jun;61(2):153-60.
The newly designed and optimized miniplex contains the following markers: D3S3053, D6S474, D9S2157, D20S482 and sex-determining marker - amelogenin. The target amplicon lengths for the developed multiplex are 71-135 bp. Amplification products were detected in a fluorescence based automated genetic analyzer. A minimal DNA sample required to obtain full genetic profiles was 250 pg. The usefulness of these miniSTRs in genotyping of severely degraded forensic samples, such as stains of blood and semen, saliva on cigarette butts and telogen hair has been confirmed in validation studies. The designed pentaplex offers a new potential screening tool in cases of old crime scenes, mass disasters, mass graves, etc., where DNA degradation, body fragmentation or large numbers of victims occur. The use of additional non-CODIS markers may increase typeability of severely degraded samples and ensure a higher potential for genetic discrimination.
D3S3053、D6S474、D9S2157、D20S482以及性别决定标记物——牙釉蛋白。所开发的复合扩增体系的目标扩增片段长度为71 - 135碱基对。扩增产物在基于荧光的自动化基因分析仪中进行检测。获得完整基因图谱所需的最小DNA样本量为250皮克。在验证研究中已证实这些微型短串联重复序列（miniSTRs）在严重降解的法医样本（如血迹、精液斑、烟蒂上的唾液和休止期毛发）基因分型中的有效性。所设计的五重复合扩增体系为旧犯罪现场、大规模灾难、乱葬岗等出现DNA降解、尸体碎片化或大量受害者的案件提供了一种新的潜在筛查工具。使用额外的非联合DNA索引系统（CODIS）标记物可能会提高严重降解样本的分型能力，并确保更高的基因鉴别潜力。
