使用为指尖采血设计的微图案纸张设备测量肝功能标志物。
Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.
机构信息
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.
出版信息
Anal Chem. 2012 Mar 20;84(6):2883-91. doi: 10.1021/ac203434x. Epub 2012 Mar 2.
Abstract
This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.
摘要
本文描述了一种基于纸张的微流控装置，该装置可测量两种肝功能酶标志物（碱性磷酸酶，ALP 和天冬氨酸氨基转移酶，AST）和总血清蛋白。该装置由四个部分组成：（i）顶部塑料片，（ii）滤膜，（iii）含有分析所需试剂的图案化纸质芯片，以及（iv）底部塑料片。该装置既能进行样品制备（将血浆与红细胞分离），也能进行测定；它还能对数据进行定性和定量分析。从纸质微流控装置获得的数据显示，校准运行和“加标”标准的标准偏差可接受，可用于常规临床使用。该装置说明了一种在资源匮乏环境中可用于多种检测的测试类型。
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2
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4
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