Dai Yanyu, Liu Zhichao, Bai Yunfeng, Chen Zezhong, Qin Jun, Feng Feng
College of Chemistry and Chemical Engineering, Shanxi University Taiyuan 030006 P. R. China
Institute for Advanced Study and Department of Chemistry, Nanchang University Nanchang 330031 P. R. China.
RSC Adv. 2018 Dec 18;8(73):42246-42252. doi: 10.1039/c8ra09298b. eCollection 2018 Dec 12.
In this study, novel highly fluorescent sulfur, nitrogen, and oxygen co-doped carbon dots (S, N, O-CDs) were prepared from -phenylenediamine and sulfamide by using the hydrothermal method. The prepared S, N, O-CDs show high doping rate and fluorescence yield as well as long-term fluorescence stability. In addition, S, N, O-CDs show good fluorescence response towards Cu over a concentration range of 2-60 μM with a detection limit of 0.29 μM. Taking advantages of the properties of S, N, O-CDs including high selectivity and low cytotoxicity, S, N, O-CDs were successfully applied for Cu sensing and imaging in the cells and O˙-induced Cu increase in the cells was observed. Furthermore, on account of strong complexation between Cu and pyrophosphate ion (PPi) as well as specific hydrolysis ability of alkaline phosphatase (ALP) towards PPi, PPi and ALP were further detected with high selectivity based on S, N, O-CDs/Cu system. The prepared S, N, O-CDs showed good detection results for PPi and ALP with detection limits of 0.44 μM and 1.03 U L, respectively. Moreover, the developed method also realized PPi and ALP detection in real samples.
在本研究中，采用水热法由对苯二胺和磺胺制备了新型高荧光硫、氮、氧共掺杂碳点（S，N，O-CDs）。所制备的S，N，O-CDs具有高掺杂率、高荧光产率以及长期荧光稳定性。此外，S，N，O-CDs在2-60 μM的浓度范围内对Cu表现出良好的荧光响应，检测限为0.29 μM。利用S，N，O-CDs具有高选择性和低细胞毒性的特性，成功将其应用于细胞中Cu的传感与成像，并观察到细胞中O˙-诱导的Cu增加。此外，由于Cu与焦磷酸根离子（PPi）之间的强络合作用以及碱性磷酸酶（ALP）对PPi的特异性水解能力，基于S，N，O-CDs/Cu体系进一步实现了对PPi和ALP的高选择性检测。所制备的S，N，O-CDs对PPi和ALP表现出良好的检测结果，检测限分别为0.44 μM和1.03 U L。此外，所建立的方法还实现了实际样品中PPi和ALP的检测。
