Parker C D, Field L H, Berry L J, Manclark C
Dev Biol Stand. 1978;41:23-9.
Differential centrifugation was used to prepare fractions from broken cells of Bordetella pertussis strain 114. Whole cells and several fractions were then assayed for potency and for safety. Crude ribosomal fractions were uniformly protective. However, ribosomes purified by washing in high salt solution and recentrifugation were at least 40 fold less potent. Protective antigen was found in the wash fluid. Wash fluid was subjected to SDS-polyacrylamide gel electrophoresis. No specific protein or carbohydrate has yet been identified as a protective immunogen. It is clear that ribosomes are not protective, but copurify with protective antigen. SDS-polyacrylamide gel analysis of soluble material purified from ribosomes may be of value in experimental studies on pertussis vaccine. If the protective immunogen can be identified, this procedure may also be of value in vaccine standardization.
采用差速离心法从百日咳博德特氏菌114株破碎细胞中制备组分。然后对全细胞和几个组分进行效力和安全性检测。粗核糖体组分具有一致的保护性。然而，用高盐溶液洗涤并再次离心纯化的核糖体效力至少低40倍。在洗涤液中发现了保护性抗原。对洗涤液进行SDS-聚丙烯酰胺凝胶电泳。尚未鉴定出任何特定的蛋白质或碳水化合物作为保护性免疫原。显然，核糖体不具有保护性，但与保护性抗原共纯化。对从核糖体中纯化的可溶性物质进行SDS-聚丙烯酰胺凝胶分析可能在百日咳疫苗的实验研究中具有价值。如果能够鉴定出保护性免疫原，该方法在疫苗标准化方面也可能具有价值。
