Immunology Research Group, Hungarian Academy of Sciences, MTA-ELTE, Pázmány P.s. 1/C, Budapest 1117, Hungary.
Cell Mol Life Sci. 2012 Aug;69(16):2717-25. doi: 10.1007/s00018-012-0947-z. Epub 2012 Mar 4.
Microarray technology outgrew the detection of simple intermolecular interactions, as incubation of slides with living cells opened new vistas. Cell-based array technology permits simultaneous detection of several different cell surface molecules, allowing the complex characterization of cells with an amount of information that is hardly assessed by any other technique. Furthermore, binding of cells to printed antibodies or ligands may induce their activation, and consequently the outcome of these interactions, such as phosphorylation, gene expression, secretion of various products; differentiation, proliferation and apoptosis of the cells are also measurable on arrays. Moreover, since cells can be transfected with printed vectors, over- or under-expression of selected genes is also achievable simultaneously, creating a nice tool for assessing the function of a given gene. The enormously high-throughput cell-based microarray technology enables testing the effect of external stimuli on a scale that was earlier unthinkable. This review summarizes the possible applications of cell-based arrays.
微阵列技术的发展已经超越了简单的分子间相互作用的检测，因为与活细胞孵育的载玻片开辟了新的视野。基于细胞的阵列技术允许同时检测几种不同的细胞表面分子，从而可以用任何其他技术都难以评估的信息量对细胞进行复杂的特征描述。此外，细胞与印刷抗体或配体的结合可能会诱导它们的激活，因此这些相互作用的结果，如磷酸化、基因表达、各种产物的分泌；细胞的分化、增殖和凋亡也可以在阵列上进行测量。此外，由于可以用印刷载体转染细胞，因此也可以同时实现选定基因的过表达或低表达，这为评估特定基因的功能提供了一个很好的工具。基于细胞的微阵列技术具有极高的高通量，可以在以前难以想象的规模上测试外部刺激对细胞的影响。这篇综述总结了基于细胞的阵列的可能应用。
