Sobek Jens, Bartscherer Kerstin, Jacob Anette, Hoheisel Jvrg D, Angenendt Philipp
Functional Genome Center Zurich, ETH Zurich/ University of Zurich, Winterhurerstrasse 190, CH- Zurich, Switzerland.
Comb Chem High Throughput Screen. 2006 Jun;9(5):365-80. doi: 10.2174/138620706777452429.
Over the last years microarray technology has become one of the principal platform technologies for the high-throughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.
在过去几年中，微阵列技术已成为生物系统高通量分析的主要平台技术之一。从20世纪90年代首次构建DNA微阵列开始，微阵列技术在过去几年蓬勃发展，并且开发出了许多不同的新形式。肽和蛋白质微阵列现在用于阐明相互作用伙伴、修饰位点和酶底物。抗体微阵列被认为在翻译谱分析方法中对蛋白质丰度的高通量测定具有高度重要性。首批细胞微阵列已经构建出来，以便将微阵列技术从体外技术转变为体内功能分析工具。所有这些方法都有一个共同的前提条件：它们所构建于其上的固体支持物。因此，对这种固体支持物的要求与应用本身一样多种多样。本综述旨在展示表面化学和衍生化方面的最新进展，并总结微阵列技术不同应用领域的最新发展情况。
