从黄褐马尾藻降解菌中鉴定内切型海藻酸钠裂解酶（Alg7D）的特性。
Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans.
机构信息
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
出版信息
Biotechnol Lett. 2012 Jun;34(6):1087-92. doi: 10.1007/s10529-012-0876-9. Epub 2012 Mar 4.
A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na(+). The K(M), V(max), k(cat), and k(cat)/K(M) values were: 3 mg ml(-1), 6.2 U mg(-1), 1.9 × 10(-2) s(-1), and 6.3 × 10(-3) mg(-1 )ml s(-1), respectively.
从裂殖壶菌(Saccharophagus degradans)中克隆得到一个编码褐藻胶裂解酶的基因 alg7D，该酶属于多糖裂解酶家族 7。alg7D 在大肠杆菌中得到了过表达。对重组 Alg7D 的性质进行了表征。该酶通过β-消除作用将褐藻胶内切解聚成聚合度为 2-5 的寡褐藻糖。其最适反应温度为 50°C，最适 pH 为 7，在 Na(+)存在下略有增加。K(M)、V(max)、k(cat)和 k(cat)/K(M)值分别为：3 mg ml(-1)、6.2 U mg(-1)、1.9 × 10(-2) s(-1)和 6.3 × 10(-3) mg(-1 )ml s(-1)。
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