Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 5445 Microbial Sciences Building, 1550 Linden Dr., Madison, WI 53706, USA.
Appl Microbiol Biotechnol. 2012 Apr;94(1):223-9. doi: 10.1007/s00253-012-3968-5. Epub 2012 Mar 6.
We have developed a simple, rapid, quantitative colorimetric assay to measure cellulose degradation based on the absorbance shift of Congo red dye bound to soluble cellulose. We term this assay "Congo Red Analysis of Cellulose Concentration," or "CRACC." CRACC can be performed directly in culture media, including rich and defined media containing monosaccharides or disaccharides (such as glucose and cellobiose). We show example experiments from our laboratory that demonstrate the utility of CRACC in probing enzyme kinetics, quantifying cellulase secretion, and assessing the physiology of cellulolytic organisms. CRACC complements existing methods to assay cellulose degradation, and we discuss its utility for a variety of applications.
我们开发了一种简单、快速、定量的比色法，用于测量基于刚果红染料与可溶性纤维素结合的吸光度变化的纤维素降解。我们将这种测定法称为“刚果红分析纤维素浓度”（Congo Red Analysis of Cellulose Concentration），简称“CRACC”。CRACC 可以直接在培养介质中进行，包括含有单糖或二糖（如葡萄糖和纤维二糖）的丰富和定义明确的培养基。我们展示了来自我们实验室的示例实验，证明了 CRACC 在探测酶动力学、量化纤维素酶分泌和评估纤维素分解生物体生理学方面的实用性。CRACC 补充了现有的测定纤维素降解的方法，我们讨论了它在各种应用中的实用性。
