Manuela Quattrocchi, Gianbattista Lobreglio, Gianpiero Leuzzi, Antonella De Donno
Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Lecce, Italy.
J Clin Med Res. 2011 Dec;3(6):291-5. doi: 10.4021/jocmr647w. Epub 2011 Nov 10.
The aim of our pilot study is to investigate different components of the immune response to influenza vaccination in a group of healthy volunteers. We evaluated the cellular immune response (CD4(+) T lymphocytes) by flow cytometry. The humoral immune response was assessed by measuring the serum haemagglutination inhibition antibody response.
Healthy adult donors (n = 18), were vaccinated with a commercially influenza virus vaccine (FLUARIX® GlaxoSmithKline S.p.a. Verona, Italy), peripheral blood was drawn the same day as influenza virus vaccination and one month later in order to enumerate the antigen-specific CD4(+) T lymphocytes. Hemagglutination inhibition assay was performed to enumerate the titer of neutralizing antibodies. Samples of nasal-pharyngeal secretions were taken by swabbing, from ILI (Influenza like Illness) subjects among the studied group, in order to verify influenza infections and eventually identify viruses using Real Time PCR.
Parenteral influenza vaccination results in significant increase in the CD4(+) Th cell population after vaccination. The number of pre-vaccination CD4(+) T cells was 0.018 [the results are presented as number of percent fluorescent cells per 10 000 lymphocytes (fixed cells)], while there was a significantly higher number of CD4(+) Th cells one month after vaccination (statistical significance was set at the level of α = 0.01). Twenty-two percent of patients demonstrated protective antibody levels to influenza A H1/N1 serotype. None was diagnosed with influenza type A or B.
T cells; Flow cytometry; Influenza virus; Influenza vaccine.
我们的初步研究旨在调查一组健康志愿者对流感疫苗接种的免疫反应的不同组成部分。我们通过流式细胞术评估细胞免疫反应（CD4(+) T淋巴细胞）。通过测量血清血凝抑制抗体反应评估体液免疫反应。
18名健康成年供体接种了一种商业流感病毒疫苗（FLUARIX®，葛兰素史克意大利维罗纳公司），在接种流感病毒疫苗的当天及一个月后采集外周血，以计数抗原特异性CD4(+) T淋巴细胞。进行血凝抑制试验以计数中和抗体滴度。通过擦拭从研究组中的流感样疾病（ILI）受试者采集鼻咽分泌物样本，以验证流感感染并最终使用实时PCR鉴定病毒。
肌肉注射流感疫苗接种后导致接种后CD4(+) Th细胞群体显著增加。接种前CD4(+) T细胞数量为0.018[结果表示为每10000淋巴细胞（固定细胞）中荧光细胞的百分比数量]，而接种后一个月CD4(+) Th细胞数量显著更高（统计学显著性设定为α = 0.01水平）。22%的患者对甲型H1/N1血清型流感表现出保护性抗体水平。无人被诊断为甲型或乙型流感。
T细胞；流式细胞术；流感病毒；流感疫苗。
