Guitton J, Desage M, Lepape A, Degoute C S, Manchon M, Brazier J L
Laboratoire d'Etudes Analytiques et Cinétiques du Médicament, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, France.
J Chromatogr B Biomed Appl. 1995 Jul 21;669(2):358-65. doi: 10.1016/0378-4347(95)00105-r.
A gas chromatographic-mass spectrometric assay, using selected-ion monitoring (GC-MS-SIM) with thymol as internal standard, was developed for quantitating propofol, an intravenous anaesthetic. The method described is rapid and sensitive for the determination of propofol in whole blood. The sensitivity of the present method is 10 ng/ml. The recovery of propofol added to human whole blood in the concentration range 10-10,000 ng/ml ranged between 95 and 100%. A single extraction procedure was used with chloroform-ethyl acetate. The assay allowed the detection of two metabolites formed during propofol metabolism: 2,6-diisopropyl-1,4-quinone and 2,6-diisopropyl-1,4-quinol.
建立了一种气相色谱 - 质谱分析法，采用以百里酚为内标的选择离子监测（GC-MS-SIM）法来定量测定静脉麻醉剂丙泊酚。所描述的方法对于全血中丙泊酚的测定快速且灵敏。本方法的灵敏度为10 ng/ml。添加到人体全血中浓度范围为10 - 10,000 ng/ml的丙泊酚回收率在95%至100%之间。使用氯仿 - 乙酸乙酯进行单次萃取程序。该分析方法能够检测丙泊酚代谢过程中形成的两种代谢产物：2,6 - 二异丙基 - 1,4 - 醌和2,6 - 二异丙基 - 1,4 - 氢醌。
