Duan Xiaopeng, He Fuyuan, Zhou Jin, Zeng Jiaoli, Xie Xianggui, Wang Haiqin
Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.
Zhongguo Zhong Yao Za Zhi. 2011 Dec;36(23):3247-52.
To study the differences of HPLC fingerprints with the total quantum statistical moment for the Buyang Huanwu decoction (BYHWD) processed by various ways, and to verify the additive properties of total quantum statistical moment.
The extracts of BYHWD were obtained by water extraction and alcohol precipitation firstly, and then were dissolved with five solvents of different solubility parameter from 11.4 Cal(1/2) x cm(-3/2) to 23.40 Cal(1/2) x cm(-3/2) respectively. Their chromatographic fingerprints were determined by HPLC, finally the total quantum statistical moment parameters and its superposition properties were manual calculated and analyzed by their expressions.
As a contrast as the whole prescription, the similarities of the various processed samples with butanol (11.4 Cal(1/2) x cm(-3/2)), methanol (13.5 Cal(1/2) x cm(-3/2)), 68% methanol (16. 67 Cal(1/2) x cm(-3/2)), 34% methanol (20.03 Cal(1/2) x cm(-3/2)) and distilled water (23.40 Cal(1/2) x cm(-3/2)) were 0. 074, 0. 973, 0. 934, 0. 991, 0. 993, respectively and while the RSD of these total quantum zero moment, center moment and variance of each chromatographic fingerprints for them were 63.04%, 16.22%, 69.38%, which showed significant difference in these chromatographic fingerprints. The total quantum statistical moment parameters of the superimposed chromatographic fingerprint with each sole samples were 3.203 x 10(5) mAu x s, 29.85 min, 389.97 min2, whereas the whole prescription's were 6.548 x 10(4) mAu x s, 29.44 min, 389.00 min2, that suggested that the absolute difference percentages between the superimposed and the whole were 2.209%, 1.389%, 0.2484%, respectively.
The total quantum statistical moment of the chromatographic fingerprints is of characteristics with additive properties, it can be used in static and dynamic quality controlled analyses in the Chinese medicine multiple component systems.
研究不同方法炮制的补阳还五汤高效液相色谱指纹图谱及总量子统计矩的差异，验证总量子统计矩的加和性。
补阳还五汤提取物先经水提醇沉，再分别用11.4 Cal(1/2) x cm(-3/2)至23.40 Cal(1/2) x cm(-3/2)五种不同溶解度参数的溶剂溶解，采用高效液相色谱法测定其色谱指纹图谱，最后通过表达式手工计算并分析总量子统计矩参数及其叠加性质。
以全方为对照，各炮制样品与正丁醇（11.4 Cal(1/2) x cm(-3/2)）、甲醇（13.5 Cal(1/2) x cm(-3/2)）、68%甲醇（16. 67 Cal(1/2) x cm(-3/2)）、34%甲醇（20.03 Cal(1/2) x cm(-3/2)）和蒸馏水（23.40 Cal(1/2) x cm(-3/2)）的相似度分别为0. 074、0. 973、0. 934、0. 991、0. 993，各色谱指纹图谱的总量子零矩、中心矩及方差的相对标准偏差分别为63.04%、16.22%、69.38%，色谱指纹图谱差异显著。各单一样品叠加后的色谱指纹图谱总量子统计矩参数为3.203 x 10(5) mAu x s、29.85 min、389.97 min2，而全方的为6.548 x 10(4) mAu x s、29.44 min、389.00 min2，叠加值与全方值的绝对差异百分比分别为2.209%、1.389%、0.2484%。
色谱指纹图谱的总量子统计矩具有加和性特征，可用于中药多成分体系的静态和动态质量控制分析。
