Liu Li-Mei, Chen Lin, Wang Rui-Hai, Yang Qing, Weng Xiao-Gang, Wang Li
Basic Theory Research Institute of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(24):2932-5.
To establish fingerprint of Cortex Fraxini and provide reference for quality evaluation of Cortex Fraxini.
Chromatographic experiments were performed on Agilent Extend C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water, containing 0.4% acetic acid as the mobile phases in gradient elution. The detection wavelength was 0-60 min, 340 nm; 6-74 min, 254 nm; 74-75 min, 340 nm, and the flow rate was 1.0 mL x min(-1). Forty samples in four varieties of Cortex Fraxini were detected to establish fingerprints, respectively.
Every parameter of the method validation complied with related rules and regulations. There were 15 common peaks in the fingerprint of 10 Fraxinus rhynchophylla samples, eleven common peaks in the fingerprint of 10 F. chinensis var. acuminata. samples, and in the fingerprint of 10 F. chinensis samples. Nineteen common peaks in the fingerprint of 10 F. stylosa samples. There were 5 common peaks in the fingerprints of 40 Cortex Fraxini samples. The similarity factors of the 10 samples of every species were all more than 0.96 compared with the control fingerprint. The similarity of the 40 Cortex Fraxini samples was more than 0.90. Four effective constituents and one unknown constituent were found in 40 samples.
The fingerprints of F. rhynchophylla bark, F. chinensis bark, F. chinensis var. acuminata bark, F. stylosa bark and Cortex Fraxini were established. The methodological evaluation showed that the results were in accord with the technology requirements of chromatography fingerprint, and it laid a good foundation for quality control of Cortex Fraxini.
建立秦皮指纹图谱，为秦皮的质量评价提供参考。
采用Agilent Extend C18柱（4.6 mm×250 mm，5μm）进行色谱实验，以甲醇和水为流动相，含0.4%乙酸，梯度洗脱。检测波长为0～60 min，340 nm；6～74 min，254 nm；74～75 min，340 nm，流速为1.0 mL·min⁻¹。分别对秦皮四个品种的40个样品进行检测以建立指纹图谱。
方法学验证的各项参数均符合相关规定。10个大叶白蜡树样品指纹图谱中有15个共有峰，10个尖叶白蜡树样品指纹图谱中有11个共有峰，10个白蜡树样品指纹图谱中有19个共有峰，10个宿柱白蜡树样品指纹图谱中有5个共有峰。40个秦皮样品指纹图谱中有5个共有峰。各品种10个样品与对照指纹图谱的相似度因子均大于0.96。40个秦皮样品的相似度大于0.90。在40个样品中发现了4种有效成分和1种未知成分。
建立了大叶白蜡树皮、白蜡树皮、尖叶白蜡树皮、宿柱白蜡树皮及秦皮的指纹图谱。方法学评价表明结果符合色谱指纹图谱的技术要求，为秦皮的质量控制奠定了良好基础。
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