Zhang Hua, An Rui, Xu Ranchi, Zhang Yizhu, Zhang Bosha, Wang Xinhong
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhongguo Zhong Yao Za Zhi. 2011 Dec;36(23):3332-7.
Using rat everted gut sacs to investigate the intestinal absorption characteristics of flavones in Gegenqinlian decoction and different compatibilities.
In the research, the intestinal absorption mechanism of the active ingredients including puerarin, daidzin, liquiritin, scutellarin, baicalin and wogonoside in Gegenqinlian decoction were studied; and then the influences of different intestinal segments, different drug concentrations on the absorption of ingredients were discussed; finally, the absorption characteristics of the active compounds in different compatibility were also be compared.
The absorption mode of the six compounds we disscussed was linearity, R2 > 0.9, which was consistent with the zero order rate process. The mechanism of absorption of puerarin, daidzin, liquiritin and scutellarin across intestinal sacs was passive diffusion, while active transportation was also existed. The Ka of baicalin and wogonoside increased along with the raising dosage, which indicated the passive absorption of the two ingredients. Different parts of the intestinal absorption experiments of Gegenqinlian decoction showed that the best absorption site of puerarin, daidzin and scutellarin was jejunum; liquiritin and baicalin can be absorbed better in colon; and the best absorption part of wogonoside was ileum. In the section of comparing the Ka of different compatibilities, it was founded that the absorption of puerarin, daidzin, liquiritin, baicalin in Gegenqinlian decoction were best; in the four intestinal segments, scutellarin and wogonoside were also absorbed best in Gegenqinlian decoction except for the duodenum where the best compatibility was Gegenqinlian group.
For ingerdients, there was selective absorption in intestinal sacs. The intestinal absorption characteristics of each component in different compatibilities were different, however, the Gegenqinlian decoction was the optimal.
采用大鼠外翻肠囊法研究葛根芩连汤及其不同配伍中黄酮类成分的肠道吸收特性。
研究葛根芩连汤中葛根素、大豆苷、甘草苷、黄芩苷、黄芩素和汉黄芩苷等活性成分的肠道吸收机制；探讨不同肠段、不同药物浓度对成分吸收的影响；最后比较不同配伍中活性成分的吸收特性。
所讨论的6种化合物的吸收模式呈线性，R2>0.9，符合零级速率过程。葛根素、大豆苷、甘草苷和黄芩苷跨肠囊的吸收机制为被动扩散，同时也存在主动转运。黄芩苷和汉黄芩苷的Ka值随剂量增加而增大，表明这两种成分的吸收为被动吸收。葛根芩连汤不同部位肠道吸收实验表明，葛根素、大豆苷和黄芩苷的最佳吸收部位为空肠；甘草苷和黄芩苷在结肠吸收较好；汉黄芩苷的最佳吸收部位为回肠。在比较不同配伍Ka值的实验中发现，葛根芩连汤中葛根素、大豆苷、甘草苷、黄芩苷的吸收最好；在四个肠段中，黄芩素和汉黄芩苷除在十二指肠中最佳配伍为葛根芩连汤组外，在葛根芩连汤中吸收也最好。
肠囊中各成分存在选择性吸收。不同配伍中各成分的肠道吸收特性不同，但葛根芩连汤为最优配伍。
