Liu Yi, Cheng Mei-Mei, Dong Jian-Jian, Zhou An
The Experimental Research Center, Anhui University of Chinese Medicine, Hefei 230038, China.
Research Institute Affiliated Hospital Neurological, Anhui University of Chinese Medicine, Hefei 230061, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jul;42(13):2571-2576. doi: 10.19540/j.cnki.cjcmm.20170523.012.
To study the intestinal absorptive characteristics of the ethanol extracts from Gandou decoction(GDD), everted intestinal sac models were used. The six representative ingredients (berberine hydrochloride, quercetin, kaempferide, rhein, chrysophanol, and aloe emodin) of GDD, were selected as the experimental targets to investigate the absorptive characteristics of various ingredients in different intestinal sections. The results showed that all six ingredients from GDD were detected in the intestinal sac, three active ingredients (berberine hydrochloride, quercetin, kaempferide) in high, medium and low doses had linear absorption properties in the small intestine segment, consistent with zero-order absorption rate; in addition, the absorption rate constant (Ka) of three components in jejunum and ileum were increased with the increase of the concentration of GDD (P<0.05), consistent with passive absorption. However, the Ka of rhein in jejunum and ileum showed little difference with the increase of dosage, suggesting a possibility of active transport mechanism. Chrysophanol and aloe-emodin were poorly absorbed in the two segments, which had not been detected in the previous time. The results suggested that the components of GDD were selectively absorbed in the intestinal sac, and the absorption characteristic of the ingredients were not exactly similar.
为研究甘豆汤乙醇提取物的肠道吸收特性，采用外翻肠囊模型。选取甘豆汤的六种代表性成分（盐酸小檗碱、槲皮素、山柰酚、大黄酸、大黄酚和芦荟大黄素）作为实验指标，考察不同肠段各成分的吸收特性。结果表明，在肠囊中检测到甘豆汤的所有六种成分，三种活性成分（盐酸小檗碱、槲皮素、山柰酚）在高、中、低剂量下在小肠段具有线性吸收特性，符合零级吸收速率；此外，空肠和回肠中三种成分的吸收速率常数（Ka）随甘豆汤浓度的增加而增加（P<0.05），符合被动吸收。然而，大黄酸在空肠和回肠中的Ka随剂量增加变化不大，提示可能存在主动转运机制。大黄酚和芦荟大黄素在这两个肠段吸收较差，在此前时间未检测到。结果提示，甘豆汤成分在肠囊中被选择性吸收，且各成分的吸收特性并不完全相似。
