Zhang Bo-sha, An Rui, Wang Yue, Wang Xin-hong
Shanghai University of TCM, Shanghai 201203, China.
Zhong Yao Cai. 2012 Sep;35(9):1460-3.
To study the intestinal dynamics characteristics of puerarin and other active ingredients in Gegenqinlian decoction.
Caco-2 cell monolayer model was used to research the transport of puerarin and baicalin, berberine hydrochloride, liquiritin when they were used in different compatibilities, meanwhile a ultra performance liquid chromato-graphic method was also developed.
The concentration of puerarin, haicalin, berberine hydrochloride, liqpiritin in cell culture medium was measured and the apparent permeahility coefficients (Papp) as well as percent absorption (%) from apical side to basolateral side were calculated. The absorption characteristics of the four components were different when they were used in different compatibilities. Furthermore the Papp sequence of puerarin in specific groups were puerarin-baicalin-berberine-liquiritin group > puerarin-baicalin group > puerarin-berberine > puerarin-liquiritin group > single puerarin (P < 0. 05).
The intestinal absorption dynamics process of the active ingredients in Gegenqinlian decoction changes as the change of prescription compatibilities. And the intestinal absorption characteristics of puerarin is enhanced by the use of other components.
研究葛根芩连汤中葛根素及其他活性成分的肠道动力学特征。
采用Caco-2细胞单层模型研究葛根素、黄芩苷、盐酸小檗碱、甘草苷在不同配伍情况下的转运情况,同时建立了超高效液相色谱法。
测定细胞培养基中葛根素、黄芩苷、盐酸小檗碱、甘草苷的浓度,并计算表观渗透系数(Papp)以及从顶侧到基底侧的吸收百分比(%)。这四种成分在不同配伍时的吸收特性不同。此外,特定组中葛根素的Papp顺序为葛根素-黄芩苷-小檗碱-甘草苷组>葛根素-黄芩苷组>葛根素-小檗碱组>葛根素-甘草苷组>单味葛根素(P<0.05)。
葛根芩连汤活性成分的肠道吸收动力学过程随方剂配伍变化而改变。且其他成分的使用增强了葛根素的肠道吸收特性。