Antić Ana, Stanojković Zoran, Macukanović-Golubović Lana, Jelić Marija
Zavod za transfuziju krvi Nis, Srbija.
Vojnosanit Pregl. 2012 Jan;69(1):22-6. doi: 10.2298/vsp1201022a.
BACKGROUND/AIM: Pathogen inactivation in blood products using riboflavin and ultraviolet (UV) light represents a proactive approach to blood safety, not only for known infectious agents but also for new ones or not yet recognized as threats to the blood supply. This method inactivates a virus, bacteria, fungus, or protozoan pathogen from the blood product without damaging its function or shelf-life. The aim of the study was to study the influence of photoinactivation using riboflavin on the concentration of coagulation factors and coagulation inhibitors in plasma that was treated before freezing.
The examination included 30 units of plasma, separated from whole blood donated by voluntary blood donors around 6 h from the moment of collection. They were treated by riboflavin (35 mL) and UV rays (6.24 J/mL, 265-370 nm) on Mirasol aparature (Caridian BCT Biotechnologies, USA) in approximate duration of 6 min. The samples for examining were taken before (K - control units) and after illumination (I - illuminated units).
Comparing the middle values of coagulation factors in the control and illuminated units we noticed their statistically significant decrease in illuminated units (p < 0.001), but the activity of coagulation ones was still in the reference range. The most sensitive coagulation factors to photoinactivation were FVIII, FIX and FXI (21.99%, 20.54% and 17.26% loss, respectively). Anticoagulant factors were better preseved than coagulation factors.
Plasma separated from whole blood donation within 6 h, treated with riboflavin and UV light within 6 h from separation and frozen at temperature below -30 degrees C within 24 h, shows good retention of pro- and anticoagulation activity.
背景/目的：使用核黄素和紫外线对血液制品进行病原体灭活是一种保障血液安全的积极方法，不仅可针对已知的感染因子，还可针对新出现的或尚未被认定为对血液供应构成威胁的感染因子。该方法可使血液制品中的病毒、细菌、真菌或原生动物病原体失活，同时不损害其功能或保质期。本研究的目的是探讨核黄素光灭活对冷冻前处理的血浆中凝血因子和凝血抑制剂浓度的影响。
检测包括30单位血浆，这些血浆来自自愿献血者在采血后约6小时分离出的全血。使用Mirasol仪器（美国Caridian BCT生物技术公司）用核黄素（35 mL）和紫外线（6.24 J/mL，265 - 370 nm）对其进行处理，处理时间约为6分钟。检测样本在光照前（K - 对照单位）和光照后（I - 光照单位）采集。
比较对照单位和光照单位中凝血因子的中位数，我们注意到光照单位中它们有统计学意义的下降（p < 0.001），但凝血因子的活性仍在参考范围内。对光灭活最敏感的凝血因子是FVIII、FIX和FXI（分别损失21.99%、20.54%和17.26%）。抗凝因子比凝血因子保存得更好。
在采血后6小时内从全血中分离出的血浆，在分离后6小时内用核黄素和紫外线处理，并在24小时内于低于 - 30摄氏度的温度下冷冻，显示出良好的促凝血和抗凝活性保留。
