Laboratory of Experimental Hypertension, University of São Paulo School of Medicine, São Paulo, Brazil.
Nutr Metab Cardiovasc Dis. 2013 May;23(5):481-6. doi: 10.1016/j.numecd.2011.10.006. Epub 2012 Mar 6.
To evaluate the effects of low or high salt intake during pregnancy on left ventricle of adult male offspring.
Low- (LS, 0.15%), normal- (NS, 1.3%) or high-salt (HS, 8% NaCl) diet was given to Wistar rats during pregnancy. During lactation all dams received NS as well as the offspring after weaning. To evaluate cardiac response to salt overload, 50% of each offspring group was fed a high-salt (hs, 4% NaCl) diet from the 21st to the 36th week of age (LShs, NShs, HShs). The remaining 50% was maintained on NS (LSns, NSns and HSns). Echocardiography was done at 20 and 30 weeks of age. Mean blood pressure (MBP), histology and left ventricular angiotensin II content (AII) were analyzed at 36 weeks of age. Interventricular septum, left ventricular posterior wall and relative wall thickness increased from the 20th to the 30th week of age only in HShs, cardiomyocyte mean volume was higher in HShs compared to NShs, LShs and HSns. AII and left ventricular fibrosis were not different among groups.
HS during pregnancy programs adult male offspring to a blood pressure and angiotensin II independent concentric left ventricular hypertrophy, with no fibrosis, in response to a chronic high-salt intake.
评估妊娠期间低盐或高盐摄入对成年雄性子代左心室的影响。
妊娠期间给予 Wistar 大鼠低盐（LS，0.15%）、正常盐（NS，1.3%）或高盐（HS，8%NaCl）饮食。哺乳期时，所有母鼠均接受 NS 喂养，断奶后其子代也接受 NS 喂养。为评估盐负荷对心脏的反应，每组子代的 50%在第 21 至 36 周时给予高盐（hs，4%NaCl）饮食（LShs、NShs、HShs），其余 50%继续给予 NS（LSns、NSns 和 HSns）。分别于第 20 周和第 30 周行超声心动图检查，于第 36 周时检测平均血压（MBP）、组织学和左心室血管紧张素 II 含量（AII）。仅在 HShs 中，从第 20 周到第 30 周时，室间隔、左心室后壁和相对室壁厚度增加，与 NShs、LShs 和 HSns 相比，HShs 中的心肌细胞平均体积更大。各组间 AII 和左心室纤维化无差异。
妊娠期间的 HS 可使成年雄性子代对慢性高盐摄入产生一种不依赖于血压和血管紧张素 II 的向心性左心室肥厚，且无纤维化。
