da Silva A Alves, de Noronha I L, de Oliveira I B, Malheiros D M, Heimann J C
Laboratory of Experimental Hypertension, University of São Paulo School of Medicine, Av. Dr. Arnaldo, 455-3rd Floor, Room 3342, 01246-903 São Paulo, SP, Brazil.
Nutr Metab Cardiovasc Dis. 2003 Jun;13(3):133-9. doi: 10.1016/s0939-4753(03)80172-2.
To study the influence of high salt intake during pregnancy and lactation on body weight, blood pressure, and the function of the renin-angiotensin system in adult rats.
Female Wistar rats received a low (0.15 NaCl), normal (1.30), or high (8% diet) salt diet. Mating occurred on the 12th week of age. From weaning, the offspring received normal salt diet. Weekly tail-cuff blood pressure and body weight measurements were performed during pregnancy and in the offspring (body weight since weaning and tail-cuff blood pressure between the 8th and the 12th week of age). Salt sensitivity of the blood pressure was evaluated and plasma renin activity determinations were performed in the 12-week-old offspring. Immunohistochemistry for renal angiotensin II was performed in the adult offspring. Renal mass and the number of glomeruli were determined. Tail-cuff blood pressure was higher in salt overloaded dams than in normal and low salt ones. In the adult offspring from the high salt dams, lower body weight, higher tail-cuff blood pressure, lower salt sensitivity in females, and increased kidney angiotensin II were observed. Plasma renin activity did not change with changes in salt intake in the adult offspring submitted to high salt environment during the perinatal period. In the offspring, renal mass and the number of glomeruli were not influenced by the dams' salt intake.
Salt overload during pregnancy and/or lactation has long-term effects on offspring's body weight and blood pressure. In addition, high salt diet during the perinatal period induced renin-angiotensin system functional disturbances in the offspring.
研究孕期及哺乳期高盐摄入对成年大鼠体重、血压及肾素-血管紧张素系统功能的影响。
雌性Wistar大鼠分别给予低（0.15%氯化钠）、正常（1.30%）或高（8%）盐饮食。在12周龄时进行交配。断奶后，子代给予正常盐饮食。在孕期及子代中（自断奶起测量体重，在8至12周龄期间测量尾套法血压）每周测量尾套法血压和体重。评估血压的盐敏感性，并在12周龄的子代中测定血浆肾素活性。对成年子代进行肾血管紧张素II的免疫组织化学检测。测定肾脏重量和肾小球数量。盐负荷过重的母鼠尾套法血压高于正常盐和低盐母鼠。在高盐母鼠的成年子代中，观察到体重较低、尾套法血压较高、雌性盐敏感性较低以及肾脏血管紧张素II增加。在围产期处于高盐环境的成年子代中，血浆肾素活性并未随盐摄入量的变化而改变。在子代中，肾脏重量和肾小球数量不受母鼠盐摄入量的影响。
孕期和/或哺乳期盐负荷过重对后代体重和血压有长期影响。此外，围产期高盐饮食可导致子代肾素-血管紧张素系统功能紊乱。
