Wolf C F, Gans H, Subramanian V A, McCoy C H
Int J Artif Organs. 1979 Mar;2(2):97-103.
The Gunn rat, congenitally lacking the conjugating enzyme glucuronyl transferase, provides an animal model for the study of bilirubin conjugation by an extracorporeal liver assist device (LAD) composed of cultured cells and artificial capillaries. Rat hepatoma cells of the H4-II-E cell line able to form conjugated bilirubin glucuronide from bilirubin were grown to tissue density by circumfusion culture of the LAD before symbiotic hemoperfusion experiments lasting up to 24 hours. Rat bile was analyzed by thin layer chromatography for bilirubin glucuronide which was formed by the device and then excreted by the rat.
先天性缺乏结合酶葡糖醛酸转移酶的冈恩大鼠，为通过由培养细胞和人工毛细血管组成的体外肝辅助装置（LAD）研究胆红素结合提供了一个动物模型。在长达24小时的共生血液灌注实验之前，通过LAD的循环灌注培养，使能够从胆红素形成结合胆红素葡糖醛酸的H4-II-E细胞系大鼠肝癌细胞生长至组织密度。通过薄层色谱法分析大鼠胆汁中由该装置形成并随后由大鼠排泄的胆红素葡糖醛酸。
