Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
ISME J. 2012 Aug;6(8):1586-601. doi: 10.1038/ismej.2012.7. Epub 2012 Mar 8.
Changes in ocean temperature and circulation patterns compounded by human activities are leading to oxygen minimum zone (OMZ) expansion with concomitant alteration in nutrient and climate active trace gas cycling. Here, we report the response of microbial eukaryote populations to seasonal changes in water column oxygen-deficiency using Saanich Inlet, a seasonally anoxic fjord on the coast of Vancouver Island British Columbia, as a model ecosystem. We combine small subunit ribosomal RNA gene sequencing approaches with multivariate statistical methods to reveal shifts in operational taxonomic units during successive stages of seasonal stratification and renewal. A meta-analysis is used to identify common and unique patterns of community composition between Saanich Inlet and the anoxic/sulfidic Cariaco Basin (Venezuela) and Framvaren Fjord (Norway) to show shared and unique responses of microbial eukaryotes to oxygen and sulfide in these three environments. Our analyses also reveal temporal fluctuations in rare populations of microbial eukaryotes, particularly anaerobic ciliates, that may be of significant importance to the biogeochemical cycling of methane in OMZs. Eukaryotic 18S rRNA gene sequences recovered from the Saanich Inlet water column on were deposited in Genbank under accession numbers HQ864863–HQ871151.
人为活动导致的海洋温度和环流模式变化正在导致氧气最小区（OMZ）扩大，伴随而来的是营养物质和气候活性痕量气体循环的改变。在这里，我们使用不列颠哥伦比亚省温哥华岛海岸的季节性缺氧峡湾萨尼奇湾作为模型生态系统，报告了微生物真核生物种群对水柱氧气缺乏季节性变化的反应。我们结合小亚基核糖体 RNA 基因测序方法和多变量统计方法，揭示了在季节性分层和更新的连续阶段中分类操作单位的变化。元分析用于确定萨尼奇湾和缺氧/硫化的卡里亚科盆地（委内瑞拉）和弗拉姆湾（挪威）之间群落组成的常见和独特模式，以显示微生物真核生物对这些三个环境中的氧气和硫化物的共同和独特反应。我们的分析还揭示了微生物真核生物稀有种群的时间波动，特别是厌氧纤毛虫，这对 OMZ 中甲烷的生物地球化学循环可能具有重要意义。从萨尼奇湾水柱中回收的真核 18S rRNA 基因序列已在 Genbank 中以 HQ864863-HQ871151 的登录号进行了存放。
