Xie Fuwei, Shang Jingjing, Guo Jizhao, Ge Zhao, Zhang Shusheng
Chemistry Department of Zhengzhou University, Zhengzhou 450052, PR China.
J Chromatogr Sci. 2012 May;50(5):387-92. doi: 10.1093/chromsci/bms016. Epub 2012 Mar 8.
A heart-cutting two-dimensional gas chromatography (GC) method was developed for the determination of nitrobenzene compounds (NBCs) in mainstream cigarette smoke. For the method, the particulate matter of cigarette smoke was extracted with cyclohexane, purified with a silica solid-phase extraction (SPE) cartridge and analyzed by heart-cutting two-dimensional GC equipped with two electron capture detectors. The heart-cutting two-dimensional GC was achieved by a single-column GC oven equipped with a microfluidic pressure balanced device (Deans switch). Two-dimensional GC was compared to single-dimensional GC and found to be clearly better for the separation of seven NBCs from a complex smoke matrix. The limits of detection ranged from 1.28 to 9.83 ng/mL, spiked recoveries were between 88.3 and 106.8% and relative standard deviation ranged from 2.79 to 12.78%. The NBCs yields of six kinds of Chinese and international cigarettes brands, which were all smoked according to two smoking protocols (International Organization for Standardization and Health Canada Intense smoking regimens), were determined and compared.
建立了一种中心切割二维气相色谱（GC）方法，用于测定主流卷烟烟气中的硝基苯化合物（NBCs）。对于该方法，卷烟烟气中的颗粒物用环己烷萃取，用硅胶固相萃取（SPE）柱净化，并用配备两个电子捕获检测器的中心切割二维GC进行分析。中心切割二维GC通过配备微流控压力平衡装置（迪恩斯切换器）的单柱GC炉实现。将二维GC与一维GC进行比较，发现其在从复杂的烟气基质中分离七种NBCs方面明显更优。检测限为1.28至9.83 ng/mL，加标回收率为88.3%至106.8%，相对标准偏差为2.79%至12.78%。测定并比较了六种中国和国际卷烟品牌按照两种吸烟方案（国际标准化组织和加拿大卫生部强烈吸烟方案）抽吸时的NBCs产率。
