Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, USA.
Nat Protoc. 2012 Mar 8;7(4):637-42. doi: 10.1038/nprot.2012.011.
Oropharyngeal candidiasis is a frequent cause of morbidity in patients with defects in cell-mediated immunity or saliva production. Animal models of this infection are important for studying disease pathogenesis and evaluating vaccines and antifungal therapies. Here we describe a simple mouse model of oropharyngeal candidiasis. Mice are rendered susceptible to oral infection by injection with cortisone acetate and then inoculated by placing a swab saturated with Candida albicans sublingually. This process results in a reproducible level of infection, the histopathology of which mimics that of pseudomembranous oropharyngeal candidiasis in humans. By using this model, data are obtained after 5-9 d of work.
口咽念珠菌病是细胞介导免疫缺陷或唾液分泌减少患者的常见发病原因。该感染的动物模型对于研究疾病发病机制以及评估疫苗和抗真菌疗法非常重要。在这里，我们描述了一种简单的口咽念珠菌病小鼠模型。通过注射醋酸考的松使小鼠易感染口腔，然后通过将含有白色念珠菌的拭子置于舌下使小鼠感染。这一过程会导致可重复的感染水平，其组织病理学类似于人类的假膜性口咽念珠菌病。使用这种模型，在 5-9 天的工作后即可获得数据。
