尼日利亚贝宁城 HIV 感染者中肠上皮细胞内原生动物的分子流行病学特征。
Molecular epidemiologic characterization of Enterocytozoon bieneusi in HIV-infected persons in Benin City, Nigeria.
机构信息
Department of Pathology, University of Benin Teaching Hospital, Nigeria.
出版信息
Am J Trop Med Hyg. 2012 Mar;86(3):441-5. doi: 10.4269/ajtmh.2012.11-0548.
Molecular characterization of Enterocytozoon bieneusi has led to better understanding of microsporidiosis transmission in humans. This study aimed to detect and genotype E. bieneusi in human immunodeficiency virus (HIV)-infected persons. Stool specimens were collected from 463 HIV-infected patients and analyzed for E. bieneusi by polymerase chain reaction (PCR) and DNA sequence analysis of the internal transcribed spacer. E. bieneusi was detected in 77 HIV patients. CD4 cell counts < 200 cells/μL was associated with E. bieneusi infection (P = 0.09). E. bieneusi was significantly associated with weight loss (P < 0.0001), diarrhea (P = 0.006), fever (P < 0.0001), not being married (P < 0.0001), and flush type of toilet (P = 0.0007). Six known genotypes of D, A, IV, CAF2, EbpA, and Peru 8 in 31, 22, 14, 2, 1, and 1 patients, respectively, five novel genotypes of E. bieneusi, and one infection with mixed genotypes were observed in this study. Three of the novel genotypes were genetically distant to the genotypes commonly found in humans.
对肠微孢子虫的分子特征进行了研究，从而更好地了解了人类微孢子虫病的传播。本研究旨在检测和基因分型人类免疫缺陷病毒（HIV）感染者体内的肠微孢子虫。采集了 463 名 HIV 感染患者的粪便标本，通过聚合酶链反应（PCR）和内部转录间隔区的 DNA 序列分析检测肠微孢子虫。在 77 名 HIV 患者中检测到肠微孢子虫。CD4 细胞计数<200 个/μL 与肠微孢子虫感染相关（P=0.09）。肠微孢子虫与体重减轻（P<0.0001）、腹泻（P=0.006）、发热（P<0.0001）、未婚（P<0.0001）和冲水式厕所（P=0.0007）显著相关。在本研究中，观察到 6 种已知基因型 D、A、IV、CAF2、EbpA 和 Peru 8，分别在 31、22、14、2、1 和 1 名患者中，5 种新的肠微孢子虫基因型和 1 种混合基因型感染。其中 3 种新的基因型与人类中常见的基因型在遗传上有很大差异。
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