Salamandane Cátia, Lobo Maria Luísa, Afonso Sónia, Xiao Lihua, Matos Olga
Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Medical Parasitology Unit, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal.
Nova School of Business and Economics, Universidade Nova de Lisboa, Carcavelos, Portugal.
Front Microbiol. 2023 Aug 4;14:1223151. doi: 10.3389/fmicb.2023.1223151. eCollection 2023.
and are etiological agents of enteric diseases characterized by diarrhea that can progress to chronicity in humans, especially in children and in immunocompromised patients. This study aims to assess the genetic pattern of and detected in vegetables and fruits commercialized in Maputo markets, Mozambique and determine their public health importance. Eight study points were sampled: a farmer zone, a wholesale, four retail markets, and two supermarkets in Maputo city, where eight types of horticultural products were purchased. Using nested-PCR methods, 2.8% (9/321) and 1.3% (4/321) of samples monitored were positive for and , respectively. Based on the analysis of the β-giardin and ITS rRNA sequences of and detected, respectively, four different sequences of (three novel sequences: BgMZ1, BgMZ2, and BgMZ3, and one known sequence) all from assemblage B and three genotypes of (two novel sequences: EbMZ4 and EbMZ5, and one known sequence: KIN-1) from group 1. These microorganisms were found and characterized for the first time in horticultural products in Maputo markets. All identified and display high genetic similarity within their β-giardin and ITS rRNA sequences, respectively, having been clustered into assemblages and genotypes with high zoonotic transmission potential. Our study may represent a relevant step in the understanding of these intestinal pathogens in association with fresh vegetables and fruits for human consumption, for a better and broader "One Health" approach.
[病原体名称1]和[病原体名称2]是肠道疾病的病原体，其特征为腹泻，在人类尤其是儿童和免疫功能低下的患者中可能发展为慢性疾病。本研究旨在评估在莫桑比克马普托市场商业化的蔬菜和水果中检测到的[病原体名称1]和[病原体名称2]的基因模式，并确定它们对公共卫生的重要性。在马普托市选取了八个研究点进行采样：一个农民区、一个批发市场、四个零售市场和两个超市，在这些地方购买了八种园艺产品。使用巢式PCR方法，监测的样本中分别有2.8%（9/321）和1.3%（4/321）对[病原体名称1]和[病原体名称2]呈阳性。基于分别对检测到的[病原体名称1]和[病原体名称2]的β-贾第虫蛋白和ITS rRNA序列的分析，[病原体名称1]有四个不同的序列（三个新序列：BgMZ1、BgMZ2和BgMZ3，以及一个已知序列）均来自B群，[病原体名称2]有三个基因型（两个新序列：EbMZ4和EbMZ5，以及一个已知序列：KIN-1）来自第1组。这些微生物首次在马普托市场的园艺产品中被发现和鉴定。所有鉴定出的[病原体名称1]和[病原体名称2]在其β-贾第虫蛋白和ITS rRNA序列内分别显示出高度的基因相似性，已被聚类到具有高人畜共患病传播潜力的群组和基因型中。我们的研究可能是朝着更好、更广泛地采用“同一健康”方法来了解与人类食用的新鲜蔬菜和水果相关的这些肠道病原体迈出的重要一步。
