实时 PCR 检测在培养物和组织中鉴定皮炎芽生菌。
Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.
机构信息
Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
出版信息
J Clin Microbiol. 2012 May;50(5):1783-6. doi: 10.1128/JCM.00310-12. Epub 2012 Mar 7.
Abstract
The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.
摘要
TaqMan 实时 PCR 检测法是从皮炎芽生菌 BAD1 基因启动子开发而来的。该检测法可识别皮炎芽生菌的所有单倍型和 6 份石蜡包埋组织中的 5 份阳性样本。检测法的灵敏度阈值为 1pg 霉菌形式的基因组 DNA 和 2CFU 的酵母形式的皮炎芽生菌。未观察到与其他真菌 DNA 的交叉反应。该检测法可在 5 小时内快速鉴定培养物和临床标本中的皮炎芽生菌。
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