Zheng H J, Tao Z H, Cheng W F, Xu M, Fang R L, Piessens W F
Guizhou Provincial Institute of Parasitic Diseases, Guiyang, People's Republic of China.
Am J Trop Med Hyg. 1990 Nov;43(5):481-8.
To evaluate the merit of antigen detection assays as a tool to monitor the efficacy of chemotherapy for lymphatic filariasis, we serially measured antigen levels in sera from jirds infected with Brugia malayi and from humans with bancroftian filariasis. Antigenemia was detected in all animals with parasitologically proven infection and was present in jirds with prepatent or occult filariasis. Antigen levels correlated with worm burdens, and progressively declined in drug-cured animals. Treatment with diethylcarbamazine (DEC) triggered a transient increase in serum levels of filarial antigens bearing the epitope recognized by the monoclonal antibody HC 11. All patients with bancroftian filariasis became amicrofilaremic within one week after DEC treatment. Antigenemia levels slowly declined over a period of several months in all but one treated individual. Forty-two months after treatment, progressively rising antigen levels are present in 10 patients. Six of these remain amicrofilaremic; in the other 4, elevated antigenemia levels preceded or were detected at the same time as recurrent parasitemia. Periodic monitoring of antigenemia levels after treatment of patients with lymphatic filariasis can be used to identify individuals who are likely to develop recurrent microfilaremia before the parasites become detectable in blood samples, thereby allowing timely retreatment.
为了评估抗原检测试验作为监测淋巴丝虫病化疗效果工具的价值，我们连续测量了感染马来布鲁线虫的沙鼠以及班氏丝虫病患者血清中的抗原水平。在所有经寄生虫学证实感染的动物中均检测到抗原血症，并且在处于潜伏期或隐匿性丝虫病的沙鼠中也存在抗原血症。抗原水平与虫负荷相关，并且在药物治愈的动物中逐渐下降。用乙胺嗪（DEC）治疗引发了带有单克隆抗体HC 11所识别表位的丝虫抗原血清水平的短暂升高。所有班氏丝虫病患者在DEC治疗后一周内均变为无微丝蚴血症。除一名接受治疗的个体外，所有患者的抗原血症水平在数月内缓慢下降。治疗42个月后，10名患者的抗原水平逐渐升高。其中6名患者仍无微丝蚴血症；在另外4名患者中，抗原血症水平升高先于复发性寄生虫血症或与之同时被检测到。对淋巴丝虫病患者治疗后进行抗原血症水平的定期监测，可用于识别那些在血液样本中可检测到寄生虫之前可能会出现复发性微丝蚴血症的个体，从而能够及时进行再次治疗。
