Weil G J, Kumar H, Santhanam S, Sethumadhavan K V, Jain D C
Am J Trop Med Hyg. 1986 May;35(3):565-70. doi: 10.4269/ajtmh.1986.35.565.
We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect soluble filarial antigen in sera collected in a Wuchereria bancrofti-endemic area in South India. Filarial antigen was detected in 38 of 38 sera from microfilaremic patients, 3 of 48 sera from amicrofilaremic patients with lymphatic pathology, and 3 of 5 sera from former microfilaria carriers with negative blood examinations 6 months or more after diethylcarbamazine therapy. One of 32 endemic control sera, 0 of 35 nonendemic sera, and 0 of 20 B. malayi sera were positive. Antigenemia was equally detectable in sera collected at night or during the day (when microfilariae are absent from the blood). Parasite antigen was also detected in the urine of patients with positive serum tests. Antibodies to circulating filarial antigen (also detected by CIEP) were absent in all but 2 antigen-positive sera but present in 22 of 45 antigen-negative sera from clinical filariasis patients and in 9 of 31 antigen-negative sera from endemic controls. Parasite antigen detection by CIEP appears to be a sensitive, specific, and practical diagnostic test for active W. bancrofti infection.
我们使用针对犬恶丝虫和马来布鲁线虫的兔抗体进行对流免疫电泳（CIEP），以检测在印度南部班氏吴策线虫流行区采集的血清中的可溶性丝虫抗原。在38例微丝蚴血症患者的38份血清中、48例无微丝蚴但有淋巴病理学改变患者的48份血清中的3份以及乙胺嗪治疗6个月或更长时间后血液检查呈阴性的5份既往微丝蚴携带者血清中的3份检测到丝虫抗原。32份流行区对照血清中的1份、35份非流行区血清中的0份以及20份马来布鲁线虫血清中的0份呈阳性。无论在夜间还是白天（血液中无微丝蚴时）采集的血清中均可检测到抗原血症。血清检测呈阳性的患者尿液中也检测到了寄生虫抗原。除2份抗原阳性血清外，所有血清中均未检测到针对循环丝虫抗原的抗体（也通过CIEP检测），但在45份临床丝虫病患者的抗原阴性血清中的22份以及31份流行区对照的抗原阴性血清中的9份中检测到了该抗体。通过CIEP检测寄生虫抗原似乎是一种用于诊断活动性班氏吴策线虫感染的敏感、特异且实用的诊断试验。
