He Songlin, Wei Yin, Fan Xu, Hu Deyu, Sreenivasan P K
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
J Clin Dent. 2011;22(6):195-9.
This randomized double-blind clinical study evaluated the antimicrobial efficacy of two mouthwashes containing (1) 0.075% cetylpyridinium chloride (CPC) + 0.05% sodium fluoride (NaF) in an alcohol-free base and (2) 0.075% CPC + 0.05% NaF in a 6% alcohol base, versus a negative control mouthwash containing 0.05% NaF in an alcohol-free base on numbers of bacteria in supragingival plaque 12 hours after a single use and 12 hours after 14 days' use.
Enrolled subjects completed a one-week washout phase prior to providing baseline samples of supragingival plaque that were analyzed for numbers of anaerobic microorganisms. Subjects were randomized to a treatment group and instructed to rinse with 20 mL of the assigned mouthwash for 30 seconds. Post-treatment microbiological analyses were conducted on plaque samples collected 12 hours after the first use of each assigned mouthwash and after completing 14 days of twice-daily use of each assigned mouthwash. Oral examinations were completed by a dentist at each sample collection to assess soft and hard tissue oral health over the course of the study.
The study enrolled 188 adults (mean age 45.78 years; age range 23-69). Subjects rinsing with the CPC-containing mouthwash realized a statistically significant (p < 0.05) reduction in numbers of supragingival anaerobic bacteria at the 12-hour evaluation after a single use. In comparison to the control mouthwash, use of the CPC mouthwash in an alcohol base resulted in a 35.3% reduction in numbers of anaerobic plaque bacteria, while the CPC mouthwash in an alcohol-free base demonstrated a 34.5% reduction. Further, the analysis after twice-daily use for 14 days indicated that the CPC mouthwash in an alcohol base demonstrated a 73.8% reduction in anaerobic plaque bacteria, while the CPC mouthwash in an alcohol-free base demonstrated a 70.9% reduction in anaerobic plaque bacteria versus the control mouthwash.
The CPC mouthwash in an alcohol-free base reduced supragingival plaque bacteria by 34.5% and 70.9% compared to the control mouthwash 12 hours after a single use and after 14 days of use, respectively. In addition, the CPC mouthwash in an alcohol base reduced supragingival bacteria by 35.3% and 73.8% compared to the control mouthwash 12 hours after a single use and after 14 days of use, respectively. There were no statistically significant differences between the CPC-containing mouthwashes at either of the post-treatment time points.
本随机双盲临床研究评估了两种含漱液的抗菌效果，一种含漱液为在无醇基质中含有0.075%西吡氯铵（CPC）+0.05%氟化钠（NaF），另一种含漱液为在6%乙醇基质中含有0.075% CPC + 0.05% NaF，将其与一种在无醇基质中含有0.05% NaF的阴性对照含漱液相比，观察单次使用12小时后及14天每天使用两次共14天后龈上菌斑中的细菌数量变化。
入选的受试者在提供龈上菌斑基线样本进行厌氧微生物数量分析之前，先完成为期一周的洗脱期。受试者被随机分为治疗组，并被指示用20 mL指定的含漱液漱口30秒。在首次使用每种指定含漱液12小时后以及完成每种指定含漱液每天两次共14天的使用后，对收集的菌斑样本进行治疗后微生物学分析。在每次样本采集时，由一名牙医完成口腔检查，以评估研究过程中口腔软硬组织的健康状况。
该研究共纳入188名成年人（平均年龄45.78岁；年龄范围23 - 69岁）。单次使用含CPC含漱液漱口的受试者在12小时评估时，龈上厌氧细菌数量有统计学意义的显著减少（p < 0.05）。与对照含漱液相比，含乙醇基质的CPC含漱液使用后厌氧菌斑细菌数量减少了35.3%，而无醇基质的CPC含漱液减少了34.5%。此外，每天两次使用14天后的分析表明，含乙醇基质的CPC含漱液与对照含漱液相比，厌氧菌斑细菌数量减少了73.8%，而无醇基质的CPC含漱液减少了70.9%。
与对照含漱液相比，无醇基质的CPC含漱液在单次使用12小时后和使用14天后，龈上菌斑细菌数量分别减少了34.5%和70.9%。此外，含乙醇基质 的CPC含漱液与对照含漱液相比，在单次使用12小时后和使用14天后，龈上细菌数量分别减少了35.3%和73.8%。在两个治疗后时间点，含CPC的含漱液之间均无统计学显著差异。
