The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 100081, PR China.
Plant Sci. 2012 May;187:69-82. doi: 10.1016/j.plantsci.2012.01.001. Epub 2012 Jan 31.
To investigate the response of cucumber seedlings to exogenous salicylic acid (SA) and gain a better understanding of SA action mechanism, we generated a proteomic profile of cucumber (Cucumis sativus L.) cotyledons treated with exogenous SA. Analysis of 1500 protein spots from each gel revealed 63 differentially expressed proteins, 59 of which were identified successfully. Of the identified proteins, 97% matched cucumber proteins using a whole cucumber protein database based on the newly completed genome established by our laboratory. The identified proteins were involved in various cellular responses and metabolic processes, including antioxidative reactions, cell defense, photosynthesis, carbohydrate metabolism, respiration and energy homeostasis, protein folding and biosynthesis. The two largest functional categories included proteins involved in antioxidative reactions (23.7%) and photosynthesis (18.6%). Furthermore, the SA-responsive protein interaction network revealed 13 key proteins, suggesting that the expression changes of these proteins could be critical for SA-induced resistance. An analysis of these changes suggested that SA-induced resistance and seedling growth might be regulated in part through pathways involving antioxidative reactions and photosynthesis.
为了研究外源水杨酸（SA）对黄瓜幼苗的响应，深入了解 SA 的作用机制，我们生成了外源 SA 处理的黄瓜（Cucumis sativus L.）子叶的蛋白质组图谱。对每张凝胶上的 1500 个蛋白点进行分析，揭示了 63 个差异表达蛋白，其中 59 个成功鉴定。利用基于本实验室新完成的基因组建立的完整黄瓜蛋白质数据库，鉴定出的蛋白质中有 97%与黄瓜蛋白质相匹配。鉴定出的蛋白质参与了各种细胞反应和代谢过程，包括抗氧化反应、细胞防御、光合作用、碳水化合物代谢、呼吸和能量稳态、蛋白质折叠和生物合成。两个最大的功能类别包括参与抗氧化反应（23.7%）和光合作用（18.6%）的蛋白质。此外，SA 响应蛋白相互作用网络揭示了 13 个关键蛋白，表明这些蛋白的表达变化可能对 SA 诱导的抗性至关重要。对这些变化的分析表明，SA 诱导的抗性和幼苗生长可能部分通过涉及抗氧化反应和光合作用的途径来调节。
