The National Engineering Research Centre for Wheat, the Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou, 450002, China.
J Proteome Res. 2012 Dec 7;11(12):6066-79. doi: 10.1021/pr300728y. Epub 2012 Nov 5.
Pretreatment with 0.5 mM salicylic acid (SA) for 3 days significantly enhanced the growth and tolerance to subsequent drought stress (PEG-6000, 15%) in wheat seedlings, manifesting as increased shoot and root dry weights, and decreased lipid peroxidation. Total proteins from wheat leaves exposed to (i) 0.5 mM SA pretreatment, (ii) drought stress, and (iii) 0.5 mM SA treatment plus drought-stress treatments were analyzed using a proteomics method. Eighty-two stress-responsive protein spots showed significant changes, of which 76 were successfully identified by MALDI-TOF-TOF. Analysis of protein expression patterns revealed that proteins associated with signal transduction, stress defense, photosynthesis, carbohydrate metabolism, protein metabolism, and energy production could by involved in SA-induced growth and drought tolerance in wheat seedlings. Furthermore, the SA-responsive protein interaction network revealed 35 key proteins, suggesting that these proteins are critical for SA-induced tolerance.
用 0.5mM 水杨酸（SA）预处理 3 天可显著增强小麦幼苗的生长和对随后干旱胁迫（PEG-6000，15%）的耐受能力，表现为增加地上部和根的干重，降低脂质过氧化。使用蛋白质组学方法分析暴露于（i）0.5mM SA 预处理、（ii）干旱胁迫和（iii）0.5mM SA 处理加干旱胁迫处理的小麦叶片中的总蛋白。82 个应激响应蛋白斑点显示出明显变化，其中 76 个通过 MALDI-TOF-TOF 成功鉴定。蛋白质表达模式分析表明，与信号转导、应激防御、光合作用、碳水化合物代谢、蛋白质代谢和能量产生相关的蛋白质可能参与了 SA 诱导的小麦幼苗生长和耐旱性。此外，SA 响应蛋白相互作用网络揭示了 35 个关键蛋白，表明这些蛋白对 SA 诱导的耐受性至关重要。
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