Laboratoire de Parasitologie-Mycologie, Centre Hospitalo-Universitaire de Nantes, Hôtel Dieu, Nantes, France.
Med Mycol. 2012 Aug;50(6):594-600. doi: 10.3109/13693786.2012.663508. Epub 2012 Mar 12.
The term phaeohyphomycosis refers to a rare group of fungal infections characterized by the presence of dark-walled hyphae or yeast-like cells in affected tissues. Herein, we report on the clinical and epidemiological characteristics of six cases of phaeohyphomycosis due to Alternaria spp. that occurred in our hospital over a 30-month period (from January 2008 to June 2010). Interestingly, whereas histopathological examinations were positive and fungal cultures yielded molds in all cases, mycological identification using conventional phenotypic methods was never possible despite prolonged incubation of the isolates. Identification of Alternaria infectoria species complex was obtained for each isolate by amplification and sequencing of the internal transcribed spacer of the ribosomal DNA (ITS rDNA). All patients had favourable outcomes following the introduction of azole-based antifungal therapy. This case series describes the clinical course of these six patients and highlights the utility of molecular identification to help in the identification of the etiologic agent when classical mycological methods have failed.
黑霉病是一组罕见的真菌感染，其特征是在受影响的组织中存在深色壁的菌丝或酵母样细胞。在此，我们报告了 6 例由交链孢霉属引起的黑霉病的临床和流行病学特征，这些病例发生在我院 30 个月期间（2008 年 1 月至 2010 年 6 月）。有趣的是，虽然组织病理学检查均为阳性，真菌培养也均培养出霉菌，但尽管延长了培养时间，常规表型方法的微生物鉴定却从未成功。通过扩增和测序核糖体 DNA（ITS rDNA）的内部转录间隔区，对每个分离株进行鉴定，鉴定为互隔交链孢霉种复合体。所有患者在引入唑类抗真菌治疗后均取得良好的转归。本病例系列描述了这 6 例患者的临床经过，并强调了分子鉴定在经典的微生物学方法失败时有助于鉴定病原体的实用性。
