Biophys J. 2012 Mar 7;102(5):L19-21. doi: 10.1016/j.bpj.2012.01.034. Epub 2012 Mar 6.
The peripheral distributions of the cardiac ryanodine receptor (RyR) and a junctional protein, junctophilin-2 (JPH2), were examined using single fluorophore localization-based super-resolution microscopy in rat ventricular myocytes. JPH2 was strongly associated with RyR clusters. Estimates of the colocalizing fraction of JPH labeling with RyR was 90% within 30 nm of RyR clusters. This is comparable to fractions estimated from confocal data (87%). Similarly, most RyRs were associated with JPH2 labeling in super-resolution images (~81% within 30 nm of JPH2 clusters). The shape of associated RyR clusters and JPH2 clusters were very similar, but not identical, suggesting that JPH2 is dispersed throughout RyR clusters and that the packing of JPH2 into junctions and the assembly of RyR clusters are tightly linked.
使用基于单荧光团定位的超分辨率显微镜，研究了大鼠心室肌细胞中心脏兰尼碱受体（RyR）和连接蛋白 junctophilin-2（JPH2）的外周分布。JPH2 与 RyR 簇强烈相关。RyR 簇附近 30nm 范围内 JPH 标记与 RyR 的共定位分数估计约为 90%。这与共聚焦数据估计的分数（87%）相当。同样，在超分辨率图像中，大多数 RyR 与 JPH2 标记相关（30nm 范围内 JPH2 簇内为 81%）。相关 RyR 簇和 JPH2 簇的形状非常相似，但不完全相同，表明 JPH2 分散在 RyR 簇中，JPH2 成结和 RyR 簇的组装紧密相连。
