Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, China.
Bioresour Technol. 2012 May;112:151-5. doi: 10.1016/j.biortech.2012.02.071. Epub 2012 Feb 22.
In this paper, the kinetics of acid-catalyzed cellulose hydrolysis in ionic liquids (ILs) was investigated by using 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) as the model IL. General kinetic equations for the formation of glucose as well as cellooligomers were constructed at a molecular level, assuming that cellulose is fully dissolved to form a homogenous solution and that the scission of the glycosidic bond occurs randomly within the cellulose chain. Experimental data were well fitted according to these equations. Variations of kinetic parameters in the presence of different water content indicated that water behaved also as a base to decrease the acidity of the reaction medium. More importantly, it offered a profile of the evolution of cellooligomers. These results provided insights into the detailed mechanisms of cellulose hydrolysis in a non-aqueous, homogenous environment and should be valuable for developing strategies to depolymerize lignocellulosic biomass.
本文以 1-丁基-3-甲基咪唑氯([Bmim]Cl)为模型离子液体，研究了酸催化纤维素在离子液体中的水解动力学。在分子水平上构建了葡萄糖和低聚糖形成的通用动力学方程，假设纤维素完全溶解形成均相溶液，糖苷键的断裂在纤维素链内随机发生。实验数据与这些方程拟合良好。在不同含水量存在的情况下，动力学参数的变化表明水也起到了碱的作用，降低了反应介质的酸度。更重要的是，它提供了低聚糖演变的概况。这些结果深入了解了非水均相环境中纤维素水解的详细机制，对于开发木质纤维素生物质解聚策略具有重要价值。
