Medizinisches Labor Bremen, Haferwende 12, D-28357 Bremen, Germany.
Methods. 2012 Apr;56(4):477-83. doi: 10.1016/j.ymeth.2012.02.007. Epub 2012 Mar 3.
The serological diagnosis of Lyme borreliosis is accomplished by the detection of IgG and IgM antibodies specific for relevant antigens of the spirochetal pathogen Borrelia burgdorferi. Instead of the usual enzyme immune assay for screening and the Western blot technique for confirmation, bead based multiplex assays with multiple simultaneously performed distinct reactions can provide quick, automatically derived and reliable results in a single run by flow cytometer technology. The broad analytical dynamic range of assay signals and the high sensitivity and specificity of the multiplex formats allow even for a reliable use in CSF based analyses for antibody specificity index in supposed neuroborreliosis. Fluorescence intensity of the bead based reactions can be transformed into quantified values as U/ml, either for each single antigen or summed up for a group of relevant key antigens. Additionally or alternatively distinct reactions of single bead populations can be transformed to Western blot band equivalents. Internal and external quality controls with the multiplex systems show characteristic data equivalent to the conventional assay formats, so that the advantages of the multiplex assays are ready for use in the routine diagnostic laboratory.
莱姆病的血清学诊断通过检测针对螺旋体病原体伯氏疏螺旋体的相关抗原的 IgG 和 IgM 抗体来完成。与常用的用于筛选的酶免疫测定法和用于确认的 Western blot 技术不同，基于珠的多重分析可以通过流式细胞术技术在单次运行中提供快速、自动得出和可靠的结果。分析信号的宽动态范围以及多重格式的高灵敏度和特异性允许即使在疑似神经莱姆病的 CSF 分析中也能可靠地使用抗体特异性指数。基于珠的反应的荧光强度可以转换为 U/ml 的定量值，无论是针对单个抗原还是针对一组相关关键抗原进行汇总。此外或替代地，可以将单个珠群的不同反应转换为 Western blot 带等价物。多重系统的内部和外部质量控制显示出与传统测定法相当的数据，因此多重分析的优势已准备好在常规诊断实验室中使用。
