A detailed surgical method for mechanical decerebration of the rat.

Surgical methods currently employed for the mechanical decerebration of a rat are only briefly described in the literature; hence, an information void exists for researchers wishing to adopt this technique successfully. Decerebration can lead to a high rate of mortality owing to cranial bleeding, particularly if the operator is inexperienced and uninformed. The illustrated methodology presented here describes, in detail, steps in the decerebration process and indicates effective approaches, such as reversible occlusion of a carotid artery, the combined use of tissue adhesive and haemostatic sponge, and perisurgical monitoring of blood pressure, by which to control blood loss and thus maintain mean postsurgical blood pressure within acceptable physiological parameters. By using this methodology, animal losses can be minimized, particularly in the early stages of adoption of the technique, ultimately reducing the numbers required for a study of this nature.